Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,008,814	C→T	A324V (GCG→GTG)	rlmL →	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,008,814	0	C	T	100.0%	138.1 / NA	38	A324V (GCG→GTG)	rlmL	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/24);  total (14/24)

TGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGG  >  NC_000913/1008736‑1008891                                                                               |                                                                              tGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTgtgc                                                                             >  1:202209/1‑81 (MQ=255)   ccGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCt                                                                            <  2:202130/80‑1 (MQ=255)      aaaGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCAt                                                                        <  1:202131/81‑1 (MQ=255)       aaGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATa                                                                       <  1:202132/81‑1 (MQ=255)            ccGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCctg                                                                  <  1:202133/81‑1 (MQ=255)            ccGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCctg                                                                  >  1:202188/1‑81 (MQ=255)                  ggCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGt                                                            >  1:202186/1‑81 (MQ=255)                   gCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTc                                                           <  2:202134/81‑1 (MQ=255)                      caGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGa                                                        >  1:202189/1‑81 (MQ=255)                        gtgtTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATc                                                      <  1:202135/81‑1 (MQ=255)                         tgtTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCa                                                     <  1:202136/81‑1 (MQ=255)                             gAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGa                                                  >  2:814784/1‑80 (MQ=255)                             gAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGa                                                  >  2:202210/1‑80 (MQ=255)                             gAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGa                                                  >  2:202181/1‑80 (MQ=255)                              aGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGaa                                                 <  2:202137/80‑1 (MQ=255)                                cAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGaaaaa                                              >  2:202197/1‑81 (MQ=255)                                 aaCCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAAc                                             >  2:202206/1‑81 (MQ=255)                                      gCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGtt                                        <  1:202138/81‑1 (MQ=255)                                       ccATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGttt                                       <  1:202139/81‑1 (MQ=255)                                        cATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtg                                      <  2:202140/81‑1 (MQ=255)                                         aTACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtgg                                     >  1:202199/1‑81 (MQ=255)                                         aTACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtgg                                     <  1:202141/81‑1 (MQ=255)                                          tACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtggt                                    >  2:202201/1‑81 (MQ=255)                                          tACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtggt                                    <  1:202142/81‑1 (MQ=255)                                            cGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtggtgg                                  <  2:202143/81‑1 (MQ=255)                                            cGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtggtg                                   <  2:202144/80‑1 (MQ=255)                                              gggAACGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCt                                <  2:202145/81‑1 (MQ=255)                                                 aaCGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTgg                              >  2:202196/1‑80 (MQ=255)                                                  aCGTCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGaa                            <  1:202146/81‑1 (MQ=255)                                                     tCTGGACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAAtct                         >  1:202194/1‑81 (MQ=255)                                                        ggACAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAAtctctc                      >  1:202202/1‑81 (MQ=255)                                                           cAGCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCttt                   <  2:202147/81‑1 (MQ=255)                                                             gCGAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGt                 >  1:202204/1‑81 (MQ=255)                                                               gAACCGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGttt               <  1:202148/81‑1 (MQ=255)                                                                  ccGGCGCTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGt            <  1:202149/81‑1 (MQ=255)                                                                       gcTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTc       <  2:202151/81‑1 (MQ=255)                                                                       gcTGATTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTCGTTTAGTGCCTc       <  1:202150/81‑1 (MQ=255)                                                                           aTTGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGc     <  2:202152/79‑1 (MQ=255)                                                                            ttGTGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCgg  <  2:202153/81‑1 (MQ=255)                                                                               |                                                                              TGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGG  >  NC_000913/1008736‑1008891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑