Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,045,600	A→G	I68I (ATT→ATC)	gfcE ←	putative exopolysaccharide export lipoprotein GfcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,045,600	0	A	G	100.0%	106.6 / NA	30	I68I (ATT→ATC)	gfcE	putative exopolysaccharide export lipoprotein GfcE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

CATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACT  >  NC_000913/1045522‑1045676                                                                               |                                                                             cATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGAt                                                                            <  1:209129/81‑1 (MQ=255)      acacATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGtt                                                                       >  1:209160/1‑81 (MQ=255)       cacaTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGttt                                                                      <  1:209130/81‑1 (MQ=255)        acaTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTg                                                                     >  2:306037/1‑81 (MQ=255)        acaTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTg                                                                     <  2:209131/81‑1 (MQ=255)        acaTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTg                                                                     >  1:209173/1‑81 (MQ=255)           tCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGgcg                                                                  >  2:209167/1‑81 (MQ=255)                    ccGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGa                                                         >  2:209170/1‑81 (MQ=255)                     cgatacgataTTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGat                                                        <  2:209132/81‑1 (MQ=255)                         acgataTTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGatcaat                                                    >  2:209166/1‑81 (MQ=255)                          cgataTTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGatcaat                                                    <  1:209133/80‑1 (MQ=255)                             tatTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATcaatcaat                                                <  1:209134/81‑1 (MQ=255)                                   tAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGc                                          <  1:209135/81‑1 (MQ=255)                                    aGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCg                                         >  2:209176/1‑81 (MQ=255)                                     gTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCg                                         <  2:209136/80‑1 (MQ=255)                                      tttGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTg                                       <  2:209137/81‑1 (MQ=255)                                           gATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACggg                                  <  2:209138/81‑1 (MQ=255)                                           gATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACggg                                  <  2:209139/81‑1 (MQ=255)                                                gctgctCCAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGga                                 >  2:209165/1‑77 (MQ=255)                                                       cAGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTg                        <  2:209140/79‑1 (MQ=255)                                                        aGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGAc                      >  2:209168/1‑80 (MQ=255)                                                        aGCTCCGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGAc                      >  2:209171/1‑80 (MQ=255)                                                             cGGGTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCAtt                  <  1:209141/79‑1 (MQ=255)                                                              gggTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTg               >  1:209142/1‑81 (MQ=255)                                                               ggTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGt              <  2:209143/81‑1 (MQ=255)                                                               ggTTAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGt              >  2:209172/1‑81 (MQ=255)                                                                  tAGCCTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTc             >  2:209169/1‑79 (MQ=255)                                                                     ccTGCGACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGt        <  1:209144/81‑1 (MQ=255)                                                                          gACATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATAc   <  1:209145/81‑1 (MQ=255)                                                                           aCATGATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACt  >  1:209174/1‑81 (MQ=255)                                                                               |                                                                             CATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACT  >  NC_000913/1045522‑1045676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑