Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,047,811	C→T	M45I (ATG→ATA)	gfcD ←	putative lipoprotein GfcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,047,811	0	C	T	100.0%	74.1 / NA	22	M45I (ATG→ATA)	gfcD	putative lipoprotein GfcD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/7);  total (15/7)

GGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAG  >  NC_000913/1047733‑1047884                                                                               |                                                                          ggTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTAt                                                                         <  2:209557/81‑1 (MQ=255)       gTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGcaa                                                                   >  2:209597/1‑81 (MQ=255)           aaCCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAAt                                                               >  2:573703/1‑81 (MQ=255)              cGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCt                                                            >  2:209584/1‑81 (MQ=255)                gTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCtgt                                                          >  2:209595/1‑81 (MQ=255)                      aTCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTgccgc                                                     >  1:209593/1‑80 (MQ=255)                          ttATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAg                                                <  2:209558/81‑1 (MQ=255)                           tATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGCTGCGCGCATTTGGTATCCGCAACAATCCTGTGCCGCCAAAGt                                               >  1:209600/1‑81 (MQ=255)                                    atttGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGc                                       >  2:209603/3‑80 (MQ=255)                                     gTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGcga                                     >  1:209601/1‑81 (MQ=255)                                     gTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGcga                                     >  2:209594/1‑81 (MQ=255)                                           gCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAAcc                               >  2:209599/1‑81 (MQ=255)                                            cTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAAccc                              <  2:209559/81‑1 (MQ=255)                                            cTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAAccc                              <  1:209560/81‑1 (MQ=255)                                                    aCCTTCCGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATc                      <  2:209561/81‑1 (MQ=255)                                                          cGGTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCggg                  >  1:209592/1‑79 (MQ=255)                                                            gTGCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAgg              <  1:209562/81‑1 (MQ=255)                                                              gCGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGt             >  2:209589/1‑80 (MQ=255)                                                               cGATGCGCGCATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTa           <  2:209563/81‑1 (MQ=255)                                                                    cgcgcATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACt      >  2:209602/1‑81 (MQ=255)                                                                      cgcATTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACtt     >  1:209598/1‑80 (MQ=255)                                                                         aTTTGGTATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAg  >  1:209596/1‑80 (MQ=255)                                                                               |                                                                          GGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAG  >  NC_000913/1047733‑1047884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑