Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,647	A→G	S65P (TCG→CCG)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,647	0	A	G	100.0%	92.1 / NA	26	S65P (TCG→CCG)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/12);  total (14/12)

AAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGT  >  NC_000913/1069571‑1069724                                                                             |                                                                              aaaGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCAt                                                                            >  2:213381/1‑80 (MQ=255)  aaGCCGCGCTGGAGCCGTGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCAtt                                                                           >  1:213382/1‑80 (MQ=255)             gAGCCGAGATAACTGGAGACGCGACCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGaaa                                                                 <  1:213344/79‑1 (MQ=255)               gCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGAtt                                                             <  2:213345/81‑1 (MQ=255)                ccGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTg                                                            <  2:213346/81‑1 (MQ=255)                   agaTAACTGGGGGCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGa                                                         <  2:213347/81‑1 (MQ=255)                    gaTAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGAt                                                        <  1:213348/81‑1 (MQ=255)                      tAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATcc                                                      <  1:213349/81‑1 (MQ=255)                               aCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCAt                                              >  2:213387/1‑80 (MQ=255)                               aCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCAt                                              >  2:213383/1‑80 (MQ=255)                                    cccgccGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAAt                                        >  2:213403/1‑81 (MQ=255)                                              ataaAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAac                              <  1:213350/81‑1 (MQ=255)                                               taaAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAaca                             >  1:213399/1‑81 (MQ=255)                                               taaAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAaca                             >  2:213388/1‑81 (MQ=255)                                                 aaGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACAcc                           <  2:213351/81‑1 (MQ=255)                                                 aaGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGA‑TGGGATCCAGCCCCATCAGAATCGGCATTAACAcc                           <  2:213352/80‑1 (MQ=255)                                                  aGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCg                          >  2:213401/1‑81 (MQ=255)                                                    aaaaaCAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTc                        >  1:213394/1‑81 (MQ=255)                                                    aaaaaCAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTc                        >  1:213389/1‑81 (MQ=255)                                                               cGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACAt             >  1:213396/1‑81 (MQ=255)                                                               cGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACAt             >  1:213393/1‑81 (MQ=255)                                                                  gCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGATTCGGCATTAACACCGTCGCGCCAAACATCGc          >  2:213398/1‑81 (MQ=255)                                                                   ccGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGcc         <  2:213353/81‑1 (MQ=255)                                                                     gATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCAc       >  1:213391/1‑81 (MQ=255)                                                                         cccGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACcgc    <  2:213354/80‑1 (MQ=255)                                                                          ccGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCgt  <  2:213355/81‑1 (MQ=255)                                                                             |                                                                              AAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGT  >  NC_000913/1069571‑1069724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑