Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,221	A→G	H296R (CAT→CGT)	opgG →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,221	0	A	G	100.0%	84.9 / NA	24	H296R (CAT→CGT)	opgG	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/11);  total (13/11)

CACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCT  >  NC_000913/1110148‑1110298                                                                          |                                                                              cACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGc                                                                         <  1:220021/80‑1 (MQ=255) cACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGc                                                                         <  2:220020/80‑1 (MQ=255) cACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCg                                                                        >  1:220057/1‑81 (MQ=255)  aCGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCgg                                                                       <  2:220022/81‑1 (MQ=255)    gACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGt                                                                      >  2:220060/1‑80 (MQ=255)    gACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGt                                                                      >  1:220048/1‑80 (MQ=255)     aCTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTca                                                                    <  2:220023/81‑1 (MQ=255)          aaCGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCagcagc                                                               >  1:220049/1‑81 (MQ=255)          aaCGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCagcagc                                                               >  1:261677/1‑81 (MQ=255)                   tctATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTcc                                                         <  1:220024/78‑1 (MQ=255)                        ccATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGaaaa                                                 >  2:220052/1‑81 (MQ=255)                         cATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAAc                                                <  1:220025/81‑1 (MQ=255)                            gCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCg                                             >  2:220051/1‑81 (MQ=255)                              cGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCa                                           <  2:220026/81‑1 (MQ=255)                                 tAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAgg                                        >  1:220055/1‑81 (MQ=255)                                  aaTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGc                                       >  2:220054/1‑81 (MQ=255)                                           tGGATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTg                              <  1:220027/81‑1 (MQ=255)                                             gATCTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGtt                            >  1:220058/1‑81 (MQ=255)                                                cTGGCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTg                           >  1:220074/1‑79 (MQ=255)                                                   gCGTCCGTTGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGc                          <  2:220028/77‑1 (MQ=255)                                                          ttGAATAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGt               >  1:220068/1‑81 (MQ=255)                                                             aaTAACCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGa             <  1:220029/80‑1 (MQ=255)                                                                aaCCCGAAACGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTtc         >  1:220056/1‑81 (MQ=255)                                                                       aaCGTTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCt  <  1:220030/81‑1 (MQ=255)                                                                          |                                                                              CACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCT  >  NC_000913/1110148‑1110298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑