Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,113,730	T→A	I51N (ATC→AAC)	yceK →	DUF1375 domain‑containing lipoprotein YceK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,113,730	0	T	A	100.0%	93.0 / NA	26	I51N (ATC→AAC)	yceK	DUF1375 domain‑containing lipoprotein YceK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/15);  total (11/15)

GGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAG  >  NC_000913/1113653‑1113803                                                                              |                                                                          ggggcaggggcaTGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCt                                                                        >  1:220776/1‑81 (MQ=255)  gggcaggggcaTGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCtt                                                                       <  1:220728/81‑1 (MQ=255)      aggggcaTGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATc                                                                   >  2:220762/1‑81 (MQ=255)               gCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATtc                                                           <  2:220729/80‑1 (MQ=255)                        attatCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGttt                                                 >  1:220767/1‑81 (MQ=255)                         ttatCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGtttt                                                >  1:220778/1‑81 (MQ=255)                            tCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGa                                             <  2:220730/81‑1 (MQ=255)                              ccGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGAt                                            <  2:220731/80‑1 (MQ=255)                                       aaTGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTActgc                                  >  1:220775/1‑81 (MQ=255)                                           ggATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGcc                              <  2:220732/81‑1 (MQ=255)                                            gATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCg                             <  1:220736/81‑1 (MQ=255)                                            gATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCg                             <  2:220735/81‑1 (MQ=255)                                            gATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCg                             <  2:220734/81‑1 (MQ=255)                                            gATGTGCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCg                             <  2:220733/81‑1 (MQ=255)                                              tgtgCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGAt                           <  1:220737/81‑1 (MQ=255)                                                 gCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGa                        >  1:220738/1‑81 (MQ=255)                                                 gCGTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGa                        >  2:220771/1‑81 (MQ=255)                                                   gTGACTCCGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACa                      <  2:220739/81‑1 (MQ=255)                                                         ccGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATtcat                 >  1:220769/1‑80 (MQ=255)                                                          cGCCTGGCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATtcatc                <  2:220740/80‑1 (MQ=255)                                                                gCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGccc         <  1:220741/81‑1 (MQ=255)                                                                gCGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGccc         <  2:220742/81‑1 (MQ=255)                                                                 cGTTATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCg        >  1:220770/1‑81 (MQ=255)                                                                   ttATGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTa      <  1:220743/81‑1 (MQ=255)                                                                     aTGTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTAt     >  2:220783/1‑80 (MQ=255)                                                                       gTCACGAACCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAg  >  1:220773/1‑81 (MQ=255)                                                                              |                                                                          GGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAG  >  NC_000913/1113653‑1113803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑