Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,183,061	G→A	A187V (GCG→GTG)	potC ←	spermidine preferential ABC transporter membrane subunit PotC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,183,061	0	G	A	100.0%	100.5 / NA	28	A187V (GCG→GTG)	potC	spermidine preferential ABC transporter membrane subunit PotC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

CATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCC  >  NC_000913/1182987‑1183138                                                                           |                                                                              cATAACTTGGTCCGGTGACGAACGAAGAAACTTCCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCaccgcc                                                                          <  1:233137/80‑1 (MQ=255) cATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCa                                                                         >  1:233199/1‑81 (MQ=255)     aCTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCcaccgccaccgc                                                                     >  1:233172/1‑81 (MQ=255)         ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTg                                                                   <  2:233139/79‑1 (MQ=255)         ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTg                                                                   <  2:233138/79‑1 (MQ=255)             cGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTg                                                             >  2:233183/1‑81 (MQ=255)                  acgaacgaAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGt                                                        >  2:233178/1‑81 (MQ=255)                    gaacgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTgg                                                      >  2:233177/1‑81 (MQ=255)                    gaacgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTgg                                                      >  2:233187/1‑81 (MQ=255)                     aacgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTgg                                                      >  1:233165/1‑80 (MQ=255)                        gaagaaACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAg                                                   >  1:233173/1‑80 (MQ=255)                            aaaCTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGa                                              <  1:233140/81‑1 (MQ=255)                               cTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGAttt                                           >  2:233182/1‑81 (MQ=255)                                tACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGAtttt                                          >  2:233193/1‑81 (MQ=255)                                          tCCATCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATg                                <  2:233141/81‑1 (MQ=255)                                             aTCGACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGt                              >  1:233176/1‑80 (MQ=255)                                                gACAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAAt                          >  2:233174/1‑81 (MQ=255)                                                  cAGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTc                        <  1:233142/81‑1 (MQ=255)                                                   aGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCg                       >  2:233186/1‑81 (MQ=255)                                                   aGGGTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCg                       >  2:233161/1‑81 (MQ=255)                                                    gggTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGc                      <  2:233143/81‑1 (MQ=255)                                                    gggTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGc                      >  1:233179/1‑81 (MQ=255)                                                     ggTAAAGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAAATCGCt                     <  1:233144/81‑1 (MQ=255)                                                         aaGCTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGc                  <  1:233145/80‑1 (MQ=255)                                                            cTTAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCg              <  2:233146/81‑1 (MQ=255)                                                              tAACACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCAcc               >  2:233180/1‑78 (MQ=255)                                                               aaCACCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCga             <  2:233147/79‑1 (MQ=255)                                                                 cacCCAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATc         >  2:233194/1‑81 (MQ=255)                                                                    ccAGCCCACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCttt      <  1:233148/81‑1 (MQ=255)                                                                        cccACCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgcc  <  2:233149/81‑1 (MQ=255)                                                                           |                                                                              CATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCC  >  NC_000913/1182987‑1183138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑