Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	153,617	T→C	S604G (AGT→GGT)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	153,617	0	T	C	100.0%	56.7 / NA	17	S604G (AGT→GGT)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/8);  total (9/8)

ACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATT  >  NC_000913/153541‑153684                                                                             |                                                                    aCGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCtt                                                                 <  1:34182/81‑1 (MQ=255)       taACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTg                                                            <  2:34183/80‑1 (MQ=255)            gACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTAt                                                       >  2:34184/1‑80 (MQ=255)             aCGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATc                                                      <  1:34185/80‑1 (MQ=255)               gcgAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAAt                                                   <  1:34186/81‑1 (MQ=255)                 gAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATAc                                                 >  1:34225/1‑81 (MQ=255)                 gAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATAc                                                 >  2:34222/1‑81 (MQ=255)                  aGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATAct                                                <  1:34187/81‑1 (MQ=255)                                    aCTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACg                              >  2:34214/1‑81 (MQ=255)                                       gctgctAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACgttg                           >  2:34221/1‑81 (MQ=255)                                        ctgctAACGTTGAGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACgtt                            <  1:34188/79‑1 (MQ=255)                                                    aGTTGGTTATTACCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAg               >  1:34218/1‑80 (MQ=255)                                                          ttattaCCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAtttt        <  2:34189/81‑1 (MQ=255)                                                            attaCCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAAttttt       <  2:34190/80‑1 (MQ=255)                                                             ttaCCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTTGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTc      >  1:34220/1‑80 (MQ=255)                                                              taCCCTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCaa    >  1:34224/1‑81 (MQ=255)                                                                cccTTAAAGTCACCGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAAtt  >  2:34226/1‑81 (MQ=255)                                                                             |                                                                    ACGCTATAACTGACGCGAGCGTTATCGCTATAGCCACTGCTGCTAACGTTGAGTTGGTTATTACCCTTAAAGTCACTGCTTATTTGAGTATCAATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATT  >  NC_000913/153541‑153684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑