Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,183,867	G→A	T193M (ACG→ATG)	potB ←	spermidine preferential ABC transporter membrane subunit PotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,183,867	0	G	A	100.0%	141.0 / NA	38	T193M (ACG→ATG)	potB	spermidine preferential ABC transporter membrane subunit PotB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/20);  total (18/20)

CCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCAACGGCT  >  NC_000913/1183789‑1183942                                                                               |                                                                            cccATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCatcatc                                                                           <  1:233305/81‑1 (MQ=255)   cATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAg                                                                         >  1:233340/1‑81 (MQ=255)   cATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAg                                                                         >  1:233335/1‑81 (MQ=255)    aTCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGt                                                                        <  1:233306/81‑1 (MQ=255)    aTCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGt                                                                        >  2:233347/1‑81 (MQ=255)    aTCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGt                                                                        <  1:233307/81‑1 (MQ=255)     tCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTg                                                                       <  1:702525/81‑1 (MQ=255)         gTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGaa                                                                    >  2:233351/1‑80 (MQ=255)           cGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGaataa                                                                 >  2:233360/1‑81 (MQ=255)                      aaCAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGAt                                                       >  2:233345/1‑80 (MQ=255)                         aGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATaa                                                     >  1:233357/1‑79 (MQ=255)                           gCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGt                                                 <  2:233308/81‑1 (MQ=255)                               aTCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTg                                              >  1:233346/1‑80 (MQ=255)                                  gCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAAc                                          >  2:233349/1‑81 (MQ=255)                                    tGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACtt                                        >  2:233359/1‑81 (MQ=255)                                     ggCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTg                                       >  2:233344/1‑81 (MQ=255)                                      gcagcaTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACtt                                        <  2:233310/79‑1 (MQ=255)                                      gcagcaTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGc                                      <  1:233309/81‑1 (MQ=255)                                       cagcaTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCt                                     <  1:233311/81‑1 (MQ=255)                                       cagcaTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCt                                     <  2:233312/81‑1 (MQ=255)                                        agcaTCACCAGCATACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTg                                    >  2:233358/1‑81 (MQ=255)                                        agcaTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTg                                    >  1:233354/1‑81 (MQ=255)                                         gcaTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTgg                                   >  1:233362/1‑81 (MQ=255)                                           aTCACCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCa                                 <  1:233313/81‑1 (MQ=255)                                              aCCAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCg                              <  2:233314/81‑1 (MQ=255)                                               ccAGCAGACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCga                             <  1:233315/81‑1 (MQ=255)                                                cagcagACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCgag                            <  1:233316/81‑1 (MQ=255)                                                 agcagACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCAcc                               >  1:233355/1‑77 (MQ=255)                                                     gACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgc                       <  2:233317/81‑1 (MQ=255)                                                     gACATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgc                       <  1:233318/81‑1 (MQ=255)                                                      aCATCCGGCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgcg                      >  1:233350/1‑81 (MQ=255)                                                            ggCAATAATTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGcgccgc                <  1:233319/81‑1 (MQ=255)                                                               aataatTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCt              >  2:233363/1‑80 (MQ=255)                                                                   atTCCCGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGc         <  2:233320/81‑1 (MQ=255)                                                                      cccGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCaa       <  2:233321/80‑1 (MQ=255)                                                                       ccGGCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCAACg     <  1:233322/81‑1 (MQ=255)                                                                          gCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCAACGGCt  >  1:233348/1‑81 (MQ=255)                                                                          gCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCAACGGCt  <  1:233324/81‑1 (MQ=255)                                                                          gCATCATCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCAACGGCt  <  1:233323/81‑1 (MQ=255)                                                                               |                                                                            CCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCCGCCTCAAGCAACGGCT  >  NC_000913/1183789‑1183942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑