Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,272	G→T	S58* (TCG→TAG)	potB ←	spermidine preferential ABC transporter membrane subunit PotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,272	0	G	T	100.0%	74.8 / NA	21	S58* (TCG→TAG)	potB	spermidine preferential ABC transporter membrane subunit PotB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/12);  total (9/12)

TCGCCAGAAACCAGGCAAACGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGAC  >  NC_000913/1184201‑1184345                                                                        |                                                                          tCGCCAGAAACCAGGCAAACGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCaa                                                                    >  1:233409/1‑79 (MQ=255)         aaCCAGGCAAACGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTc                                                           >  2:233420/1‑80 (MQ=255)         aaCCAGGCAAACGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCa                                                          <  1:233385/81‑1 (MQ=255)            cAGGCAAACGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCaaaa                                                       >  1:233423/1‑81 (MQ=255)                 aaaCGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATagag                                                  <  2:233386/81‑1 (MQ=255)                       gTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATc                                            >  1:233424/1‑81 (MQ=255)                           cccAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGc                                        <  2:233387/81‑1 (MQ=255)                            ccAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAg                                         >  2:233427/1‑79 (MQ=255)                            ccAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAg                                         >  1:233421/1‑79 (MQ=255)                             cAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAg                                         >  1:233426/1‑78 (MQ=255)                                            gAGGGTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATc                       <  1:233388/81‑1 (MQ=255)                                               ggTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAg                    <  1:233390/81‑1 (MQ=255)                                               ggTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAg                    <  1:233389/81‑1 (MQ=255)                                                gTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGc                   <  1:233392/81‑1 (MQ=255)                                                gTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGc                   <  2:233391/81‑1 (MQ=255)                                                gTGGCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGc                   >  2:233422/1‑81 (MQ=255)                                                   gCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGt                 <  1:233394/80‑1 (MQ=255)                                                   gCGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGt                 <  1:233393/80‑1 (MQ=255)                                                    cGATCAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTaa               <  2:233395/81‑1 (MQ=255)                                                        cAGCGCCATATTCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGAc           >  2:233429/1‑81 (MQ=255)                                                                  ttCAGCTAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGAc  <  2:233396/80‑1 (MQ=255)                                                                        |                                                                          TCGCCAGAAACCAGGCAAACGGGTAGCCCAGCACCAGGCAGGCGAGGGTGGCGATCAGCGCCATATTCAGCGAGTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGAC  >  NC_000913/1184201‑1184345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑