Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,238,385	T→G	L272R (CTG→CGG)	dadA →	D‑amino acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,238,385	0	T	G	100.0%	64.3 / NA	19	L272R (CTG→CGG)	dadA	D‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/11);  total (8/11)

GATGCGTATGTGATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCTGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAACC  >  NC_000913/1238309‑1238458                                                                             |                                                                          gATGCGTATGTGATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGa                                                                         >  2:241566/1‑79 (MQ=255)        aTGTGATGGCGATTGGTTCTTACTCGACGGCGATGCCCAAAGGCATTGCTCATAATTAGGTGTATCCGCGGAAAGGCTACt                                                                >  2:241604/1‑81 (MQ=255)         tgtgATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTc                                                               <  1:241527/81‑1 (MQ=255)         tgtgATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTc                                                               <  2:241528/81‑1 (MQ=255)           tgATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGc                                                             <  2:241529/81‑1 (MQ=255)             aTGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTg                                                           <  1:241530/81‑1 (MQ=255)              tGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGa                                                          <  2:241531/81‑1 (MQ=255)                   tttGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCAtt                                                     <  2:241532/81‑1 (MQ=255)                      ggTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTcc                                                   <  1:241533/80‑1 (MQ=255)                          cTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAAtt                                               <  1:241534/80‑1 (MQ=255)                             aCTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTgcg                                            >  1:241560/1‑80 (MQ=255)                               tCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAg                                         >  1:241559/1‑81 (MQ=255)                                 gACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAgg                                        <  1:241535/80‑1 (MQ=255)                                      cGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATg                                  >  2:241568/1‑81 (MQ=255)                                              aaaGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGcc                           <  2:241536/80‑1 (MQ=255)                                                aGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCgg                         >  2:241563/1‑80 (MQ=255)                                                   cATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATc                     >  2:241561/1‑81 (MQ=255)                                                                      tATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAAcc  <  1:241537/81‑1 (MQ=255)                                                                       aTCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAAcc  >  2:241572/1‑80 (MQ=255)                                                                             |                                                                          GATGCGTATGTGATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCTGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAACC  >  NC_000913/1238309‑1238458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑