Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,239,692	T→C	Y272H (TAC→CAC)	dadX →	alanine racemase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,239,692	0	T	C	100.0%	119.7 / NA	35	Y272H (TAC→CAC)	dadX	alanine racemase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

AGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACGGTGGG  >  NC_000913/1239616‑1239768                                                                             |                                                                             aGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGc                                                                          >  2:241853/1‑81 (MQ=255) aGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGc                                                                          >  1:241851/1‑81 (MQ=255)        gAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGt                                                                   <  1:241800/81‑1 (MQ=255)        gAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGt                                                                   >  1:241850/1‑81 (MQ=255)        gAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGt                                                                   >  1:241869/1‑81 (MQ=255)           cGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTAt                                                                <  2:241801/81‑1 (MQ=255)            gtgtGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATc                                                               >  2:241855/1‑81 (MQ=255)               tgGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCcg                                                             <  2:241802/80‑1 (MQ=255)                   cTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCa                                                        >  2:241859/1‑81 (MQ=255)                      cggcggTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCAcg                                                      <  1:241803/80‑1 (MQ=255)                       ggcggTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCAcgcg                                                    >  2:241847/1‑81 (MQ=255)                          ggTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGcc                                                  >  2:241872/1‑80 (MQ=255)                           gTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTa                                                <  2:241804/81‑1 (MQ=255)                              gCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTAcc                                              <  1:241806/80‑1 (MQ=255)                              gCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTAcc                                              <  2:241807/80‑1 (MQ=255)                              gCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCg                                             <  2:241805/81‑1 (MQ=255)                                   aCTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTAcc                                        >  2:241862/1‑81 (MQ=255)                                       cgcgcgATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCt                                     <  1:241808/80‑1 (MQ=255)                                        gcgcgATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGt                                   >  1:241857/1‑81 (MQ=255)                                         cgcgATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGtt                                  <  1:241809/81‑1 (MQ=255)                                            gATGAACAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTa                               <  2:241810/81‑1 (MQ=255)                                                aaCAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTgg                           <  2:241811/81‑1 (MQ=255)                                                aaCAGCGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTg                            <  2:241812/80‑1 (MQ=255)                                                     cGAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGc                      <  1:241813/81‑1 (MQ=255)                                                      gAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCg                     >  2:241860/1‑81 (MQ=255)                                                      gAATCGGCATTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCg                     <  1:241814/81‑1 (MQ=255)                                                              aTTGTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCAcc             <  2:241815/81‑1 (MQ=255)                                                                 gTCGCCGCAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCAt           >  1:241871/1‑80 (MQ=255)                                                                    gccgcAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACg       >  1:241879/1‑81 (MQ=255)                                                                    gccgcAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACg       <  1:241816/81‑1 (MQ=255)                                                                        cAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACGGTgg   <  2:241817/81‑1 (MQ=255)                                                                        cAGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACGGTgg   >  2:241867/1‑81 (MQ=255)                                                                         aGGGCACGTAGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGAAGGCGTGCGCACCATGACGGTggg  >  2:241866/1‑81 (MQ=255)                                                                         aGGGCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACGGTggg  >  2:241865/1‑81 (MQ=255)                                                                          gggCACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACGGTggg  >  2:241861/1‑80 (MQ=255)                                                                             |                                                                             AGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCATGACGGTGGG  >  NC_000913/1239616‑1239768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑