Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,004	A→T	I270N (ATC→AAC)	ychF ←	redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,004	0	A	T	100.0%	88.7 / NA	25	I270N (ATC→AAC)	ychF	redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/11);  total (14/11)

GAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGG  >  NC_000913/1256927‑1257079                                                                              |                                                                            gAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTca                                                                          >  2:244696/1‑81 (MQ=255)  aaTGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTca                                                                          >  2:244697/1‑80 (MQ=255)      gTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACgg                                                                     >  1:244699/1‑81 (MQ=255)           tGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAg                                                                <  1:244660/81‑1 (MQ=255)           tGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAg                                                                >  1:244688/1‑81 (MQ=255)                      cttTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGctct                                                      <  2:244661/80‑1 (MQ=255)                         tCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCtt                                                     <  2:244662/78‑1 (MQ=255)                         tCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTc                                                    >  2:244703/1‑79 (MQ=255)                            ccccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGcc                                               <  2:244663/81‑1 (MQ=255)                             cccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGccc                                              <  1:244664/81‑1 (MQ=255)                               cAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGccc                                              >  1:244707/1‑79 (MQ=255)                               cAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCaa                                            <  1:244665/81‑1 (MQ=255)                                             gtTTGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAAc                              <  1:244666/81‑1 (MQ=255)                                              tttGCAGGTTCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACt                             >  1:244689/1‑81 (MQ=255)                                                      ttCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGt                     >  1:244704/1‑81 (MQ=255)                                                       tCAGCAGTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGtt                    >  2:244706/1‑81 (MQ=255)                                                         agcagTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGttct                  <  1:244667/81‑1 (MQ=255)                                                          gcagTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGttctt                 <  2:244668/81‑1 (MQ=255)                                                          gcagTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGttctt                 >  1:244695/1‑81 (MQ=255)                                                            agTTTATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTtcg               >  1:244718/1‑81 (MQ=255)                                                              tttATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTtcgtc             <  2:244669/81‑1 (MQ=255)                                                              tttATAACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTtcgtc             <  2:244670/81‑1 (MQ=255)                                                                  taACCGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCgtcgtc          >  1:244700/1‑80 (MQ=255)                                                                     ccGGCACGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGt      >  2:244701/1‑81 (MQ=255)                                                                         cacggttcacacggttcaGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCgg  >  1:244692/1‑81 (MQ=255)                                                                          aCGGTTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCgg  >  2:244705/1‑80 (MQ=255)                                                                              |                                                                            GAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGG  >  NC_000913/1256927‑1257079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑