Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,291	A→C	Q17P (CAG→CCG)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,291	0	A	C	100.0%	143.6 / NA	39	Q17P (CAG→CCG)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/21);  total (18/21)

TTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTG  >  NC_000913/1290214‑1290364                                                                              |                                                                          ttGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGt                                                                        <  1:250512/81‑1 (MQ=255) ttGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGt                                                                        <  2:250513/81‑1 (MQ=255)  tGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTa                                                                       >  1:250561/1‑81 (MQ=255)   gAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAt                                                                      >  2:250556/1‑81 (MQ=255)   gAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAt                                                                      >  1:250548/1‑81 (MQ=255)    aGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAt                                                                      <  1:250514/80‑1 (MQ=255)     gTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAttt                                                                    <  2:250515/81‑1 (MQ=255)     gTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAttt                                                                    >  1:250554/1‑81 (MQ=255)      tAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtc                                                                   <  2:250516/81‑1 (MQ=255)       aaaGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcg                                                                  >  2:250565/1‑81 (MQ=255)        aaGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcgc                                                                 <  2:250517/81‑1 (MQ=255)          gCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcgctc                                                               <  1:250518/81‑1 (MQ=255)            cAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcgctcgc                                                             >  2:250550/1‑81 (MQ=255)                 aGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTg                                                        >  2:250555/1‑81 (MQ=255)                   gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGa                                                      <  2:250519/81‑1 (MQ=255)                      agagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGAtt                                                    >  2:250560/1‑80 (MQ=255)                      agagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTc                                                   <  1:250520/81‑1 (MQ=255)                       gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCa                                                  <  1:250521/81‑1 (MQ=255)                         gaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATg                                                >  1:250552/1‑81 (MQ=255)                               gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTc                                          >  2:250573/1‑81 (MQ=255)                               gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTc                                          >  1:250549/1‑81 (MQ=255)                                aCGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTc                                          <  1:250522/80‑1 (MQ=255)                                 cGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCt                                        <  1:250523/81‑1 (MQ=255)                                  gCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTc                                       <  1:250524/81‑1 (MQ=255)                                    aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCAt                                     <  1:250526/81‑1 (MQ=255)                                    aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCAt                                     <  1:250525/81‑1 (MQ=255)                                      ccATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTg                                   <  1:250527/81‑1 (MQ=255)                                           ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc                              >  1:250571/1‑81 (MQ=255)                                            gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc                              <  1:250529/80‑1 (MQ=255)                                            gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc                              <  1:250528/80‑1 (MQ=255)                                                   aaCAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACgg                      <  1:250530/81‑1 (MQ=255)                                                     cAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTa                    >  2:250531/1‑81 (MQ=255)                                                       gATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACt                  <  1:250532/81‑1 (MQ=255)                                                             cATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTggcggc            >  1:250566/1‑81 (MQ=255)                                                              aTTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt           <  2:250533/81‑1 (MQ=255)                                                              aTTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt           >  2:250572/1‑81 (MQ=255)                                                               ttgttgAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt           >  1:250567/1‑80 (MQ=255)                                                                 gttgAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt        >  2:250568/1‑81 (MQ=255)                                                                        aTGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg  >  1:250569/1‑80 (MQ=255)                                                                              |                                                                          TTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTG  >  NC_000913/1290214‑1290364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑