Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,472	C→T	V97V (GTC→GTT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,472	0	C	T	100.0%	97.7 / NA	27	V97V (GTC→GTT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

GCTTACTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCT  >  NC_000913/1301396‑1301548                                                                             |                                                                             gCTTACTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGa                                                                          >  2:252357/1‑81 (MQ=255)  cTTACTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGAc                                                                         <  1:252319/81‑1 (MQ=255)     aCTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCtt                                                                      >  1:252360/1‑81 (MQ=255)     aCTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCt                                                                       <  1:252320/80‑1 (MQ=255)         gTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTcc                                                                    <  1:252321/79‑1 (MQ=255)             gCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGc                                                              <  2:252322/81‑1 (MQ=255)             gCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGc                                                              >  1:252361/1‑81 (MQ=255)                   ttGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTa                                                           >  1:252365/1‑78 (MQ=255)                      aCGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGAt                                                      <  2:252323/80‑1 (MQ=255)                            aTCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAAt                                               >  2:252358/1‑81 (MQ=255)                               cAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGt                                            >  2:252355/1‑81 (MQ=255)                                       ggCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATgg                                     >  1:252364/1‑80 (MQ=255)                                         cGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCAc                                  >  1:252375/1‑81 (MQ=255)                                                  atacTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTcac                         <  2:252324/78‑1 (MQ=255)                                                       gggATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGca                     >  1:252376/1‑80 (MQ=255)                                                        ggATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGcac                    <  1:252325/80‑1 (MQ=255)                                                         gATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGcac                    >  1:252362/1‑79 (MQ=255)                                                         gATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACaa                  <  1:252326/81‑1 (MQ=255)                                                              taaAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACtt             <  2:252327/81‑1 (MQ=255)                                                               aaaGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACttt            >  1:252363/1‑81 (MQ=255)                                                                  gACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTtgtg         >  1:252367/1‑81 (MQ=255)                                                                     gcgAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGta       >  2:252373/1‑80 (MQ=255)                                                                       gAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAg    >  1:252366/1‑81 (MQ=255)                                                                       gAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGAAGGCACGCCAGTCACAGCACAAGACTTTGTGTATAg    >  1:252377/1‑81 (MQ=255)                                                                         aaGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCt  <  2:252328/81‑1 (MQ=255)                                                                         aaGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCt  <  1:252330/81‑1 (MQ=255)                                                                         aaGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCt  <  1:252329/81‑1 (MQ=255)                                                                             |                                                                             GCTTACTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCT  >  NC_000913/1301396‑1301548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑