Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,311,270	T→C	V61A (GTT→GCT)	tonB →	Ton complex subunit TonB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,311,270	0	T	C	100.0%	85.0 / NA	24	V61A (GTT→GCT)	tonB	Ton complex subunit TonB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

CCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAG  >  NC_000913/1311203‑1311341                                                                    |                                                                        cctgcgcctgcgCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCg                                                            >  2:253911/1‑81 (MQ=255)    gcgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAg                                                         >  2:253921/1‑81 (MQ=255)     cgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGa                                                          <  1:253883/79‑1 (MQ=255)          gcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCggtg                                                   >  2:253919/1‑81 (MQ=255)            gcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCggtgg                                                  >  2:253920/1‑80 (MQ=255)                  gATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGcc                                           <  1:253884/81‑1 (MQ=255)                    tttCTGTCACGATGGTTACGCCGGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAg                                         <  2:253885/81‑1 (MQ=255)                          tCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAAcc                                     <  2:253886/79‑1 (MQ=255)                           cACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCgg                                   >  2:253925/1‑80 (MQ=255)                            aCGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGaa                                 >  1:253917/1‑81 (MQ=255)                             cGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAc                                <  2:253887/81‑1 (MQ=255)                              gATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAcc                               <  1:253888/81‑1 (MQ=255)                               aTGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCt                              >  2:253918/1‑81 (MQ=255)                                tGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTg                             <  2:253889/81‑1 (MQ=255)                                  gTTACGCCTGCTGATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAg                           <  1:253890/81‑1 (MQ=255)                                            ctgATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGGGCCGATcccc                  >  2:253923/1‑80 (MQ=255)                                              gATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGaa               >  1:253937/1‑81 (MQ=255)                                              gATCTCGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGaa               >  1:253924/1‑81 (MQ=255)                                               ttctcGACCCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGaa               <  1:253891/79‑1 (MQ=255)                                                  tcGAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGc           >  1:253929/1‑81 (MQ=255)                                                    gAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAAcc             <  2:253892/77‑1 (MQ=255)                                                    gAACCGCCACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGcc          >  2:253922/1‑80 (MQ=255)                                                          ccACAAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaa   <  1:253893/81‑1 (MQ=255)                                                            acaAGCCGCTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaag  >  2:253926/1‑80 (MQ=255)                                                                    |                                                                        CCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAG  >  NC_000913/1311203‑1311341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑