Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,319,991	G→A	P465L (CCG→CTG)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,319,991	0	G	A	100.0%	94.5 / NA	27	P465L (CCG→CTG)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/17);  total (10/17)

TTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTT  >  NC_000913/1319913‑1320064                                                                               |                                                                          ttCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAgt                                                                         <  2:255187/81‑1 (MQ=255)   cGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAgtg                                                                        <  2:255188/80‑1 (MQ=255)           cGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTg                                                               <  2:255189/81‑1 (MQ=255)             gctgctTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGc                                                              >  2:255233/1‑80 (MQ=255)                 ctTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGtt                                                         <  1:255191/81‑1 (MQ=255)                 ctTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGt                                                          <  2:255190/80‑1 (MQ=255)                    cATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTg                                                        <  2:255192/79‑1 (MQ=255)                     aTGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCt                                                      <  1:255193/80‑1 (MQ=255)                          cggcgTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCtggt                                                <  2:255194/81‑1 (MQ=255)                                 gCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAggg                                         >  2:255261/1‑81 (MQ=255)                                 gCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAgg                                          <  2:255195/80‑1 (MQ=255)                                   cTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGgtg                                       <  1:255196/81‑1 (MQ=255)                                   cTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGgtg                                       >  2:255236/1‑81 (MQ=255)                                         cTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGc                                 <  1:255197/81‑1 (MQ=255)                                          ttGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGc                                 >  2:255235/1‑80 (MQ=255)                                            gTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGcc                                <  2:255198/79‑1 (MQ=255)                                                  aaCGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGtctt                        >  1:255227/1‑81 (MQ=255)                                                    cGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCTAAAGtcttct                      <  2:255199/81‑1 (MQ=255)                                                     gtgtTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTg                     >  2:255230/1‑81 (MQ=255)                                                        ttAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCgg                  <  1:255200/81‑1 (MQ=255)                                                           aaaTGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGt                 >  1:255247/1‑79 (MQ=255)                                                           aaaTGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTg                <  1:255201/80‑1 (MQ=255)                                                               gTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCt            >  1:255244/1‑80 (MQ=255)                                                                tCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTg           <  1:255202/80‑1 (MQ=255)                                                                    ggTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGc      >  1:255231/1‑81 (MQ=255)                                                                     gTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCt     >  2:255241/1‑81 (MQ=255)                                                                         tcttcCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCtttt  <  2:255203/80‑1 (MQ=255)                                                                               |                                                                          TTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTT  >  NC_000913/1319913‑1320064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑