Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,321,337	G→A	N16N (AAC→AAT)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,321,337	0	G	A	100.0%	100.7 / NA	28	N16N (AAC→AAT)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

TTCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGT  >  NC_000913/1321259‑1321413                                                                               |                                                                             ttCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGAtt                                                                            <  1:255444/81‑1 (MQ=255)   cAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTg                                                                           >  1:255489/1‑80 (MQ=255)   cAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTg                                                                           <  2:255445/80‑1 (MQ=255)         aGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTaa                                                                    <  1:255446/81‑1 (MQ=255)          ggTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTaa                                                                    >  2:255484/1‑80 (MQ=255)              tGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAaga                                                                <  2:255447/80‑1 (MQ=255)               gcgcCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGt                                                              >  1:255480/1‑81 (MQ=255)                 gcCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTc                                                             >  2:255479/1‑80 (MQ=255)                   cGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGa                                                           >  1:255485/1‑80 (MQ=255)                    ggAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGata                                                         >  2:255487/1‑81 (MQ=255)                    ggAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGata                                                         >  1:255482/1‑81 (MQ=255)                      aaTATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGatat                                                        >  2:255490/1‑80 (MQ=255)                       atatGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGAtatta                                                      >  1:255495/1‑81 (MQ=255)                       atatGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGAtatta                                                      >  1:255496/1‑81 (MQ=255)                         atGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATc                                                    >  2:255486/1‑81 (MQ=255)                          tGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCg                                                   <  1:409543/81‑1 (MQ=255)                          tGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCg                                                   <  2:255448/81‑1 (MQ=255)                                cGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGagcag                                              <  2:255449/80‑1 (MQ=255)                                  gTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGcagcag                                           <  2:255450/81‑1 (MQ=255)                                    aaaTCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTATGTAAAAGAGTCGATATTATCGAGCAGCAGaa                                         >  2:255483/1‑81 (MQ=255)                                       tCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGt                                      <  1:255451/81‑1 (MQ=255)                                         accacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTc                                     <  1:255452/80‑1 (MQ=255)                                           cacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGc                                  <  2:255453/81‑1 (MQ=255)                                                   gCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGa                           >  1:255493/1‑80 (MQ=255)                                                       aTTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtc                      <  1:255454/81‑1 (MQ=255)                                                            tgcGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCtg                 >  1:255494/1‑81 (MQ=255)                                                                 aaCTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCat            <  1:255455/81‑1 (MQ=255)                                                                           ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGt  <  1:255456/81‑1 (MQ=255)                                                                               |                                                                             TTCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGT  >  NC_000913/1321259‑1321413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑