Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,437	A→T	T64T (ACT→ACA)	sapD ←	putrescine ABC exporter ATP binding protein SapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,437	0	A	T	100.0%	70.8 / NA	20	T64T (ACT→ACA)	sapD	putrescine ABC exporter ATP binding protein SapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/14);  total (6/14)

ATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAC  >  NC_000913/1353362‑1353510                                                                            |                                                                          aTGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTaa                                                                       <  2:260789/80‑1 (MQ=255)  tGGCCAACCAGTTTGCGCCGTTCGCGTGCGGGGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAaca                                                                     <  2:260790/81‑1 (MQ=255)    gCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACg                                                                   <  1:260791/81‑1 (MQ=255)     ccAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGc                                                                  <  1:260792/81‑1 (MQ=255)       aaCCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCa                                                                <  1:260793/81‑1 (MQ=255)             tttGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATc                                                          <  1:260794/81‑1 (MQ=255)               tGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCt                                                         <  2:260795/80‑1 (MQ=255)                      ttCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAc                                                 <  1:260796/81‑1 (MQ=255)                                   agaCGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCt                                    >  2:260827/1‑81 (MQ=255)                                              aTCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCaa                         <  1:260797/81‑1 (MQ=255)                                                  atatCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAActc                     <  1:260798/81‑1 (MQ=255)                                                          aaaaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCgg              <  2:260799/80‑1 (MQ=255)                                                             aCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAcc          >  1:260825/1‑81 (MQ=255)                                                             aCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAcc          <  2:260800/81‑1 (MQ=255)                                                                cATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGa       >  1:260829/1‑81 (MQ=255)                                                                 aTACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGAt      >  2:260824/1‑81 (MQ=255)                                                                  tACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGAtt     <  1:260801/81‑1 (MQ=255)                                                                   aCGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGAtt     >  2:260822/1‑80 (MQ=255)                                                                     ggTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAc  <  1:260802/81‑1 (MQ=255)                                                                      gTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAc  >  2:260826/1‑80 (MQ=255)                                                                            |                                                                          ATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAC  >  NC_000913/1353362‑1353510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑