Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,367,811	G→A	I34I (ATC→ATT)	pspF ←	DNA‑binding transcriptional dual regulator PspF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,367,811	0	G	A	100.0%	88.9 / NA	25	I34I (ATC→ATT)	pspF	DNA‑binding transcriptional dual regulator PspF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/16);  total (9/16)

AACGGCCCTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTCACCAAG  >  NC_000913/1367734‑1367888                                                                              |                                                                              aaCGGCCCTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCgataat                                                                             >  2:263569/1‑80 (MQ=255) aaCGGCCCTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGataatg                                                                            <  1:263534/81‑1 (MQ=255)     gCCCTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCa                                                                        >  1:263559/1‑81 (MQ=255)       ccTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCAcc                                                                      <  2:263536/81‑1 (MQ=255)       ccTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCAcc                                                                      <  2:263535/81‑1 (MQ=255)           gCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGt                                                                   <  1:263537/80‑1 (MQ=255)             cAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTg                                                                >  2:263567/1‑81 (MQ=255)                cGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTcc                                                             <  1:263538/81‑1 (MQ=255)                 gggAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTc                                                              >  2:263573/1‑79 (MQ=255)                             aaTGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGAt                                                >  2:263571/1‑81 (MQ=255)                                 cAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGa                                            <  1:263539/81‑1 (MQ=255)                                  aGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGaa                                           <  2:263540/81‑1 (MQ=255)                                  aGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGaa                                           <  1:263541/81‑1 (MQ=255)                                     cggcTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAAcc                                        >  1:263579/1‑81 (MQ=255)                                        cTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGt                                     <  1:263542/81‑1 (MQ=255)                                           gCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGtt                                    <  1:263543/79‑1 (MQ=255)                                            cAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTc                                   >  2:263574/1‑79 (MQ=255)                                            cAATCAGCTCTTTACCGGTGCCGCGTTCGCCGAAAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTc                                   >  2:263575/1‑79 (MQ=255)                                                 aGCTCTTTACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACt                            <  2:263544/81‑1 (MQ=255)                                                      tttACCGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCg                         <  1:263545/79‑1 (MQ=255)                                                          ccGGTGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAg                   <  1:263546/81‑1 (MQ=255)                                                              tGCCGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGt               <  2:263547/81‑1 (MQ=255)                                                                ccGCGTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTc             <  2:263548/81‑1 (MQ=255)                                                                  gcgTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGc           <  2:263549/81‑1 (MQ=255)                                                                    gTTCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCt         >  2:263576/1‑81 (MQ=255)                                                                     ttCGCCGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTc        <  2:263550/81‑1 (MQ=255)                                                                         ccGATAATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTCAcc     >  1:263578/1‑80 (MQ=255)                                                                           gataatGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTCACCAAg  <  2:263551/81‑1 (MQ=255)                                                                              |                                                                              AACGGCCCTTGCCAACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTCACCAAG  >  NC_000913/1367734‑1367888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑