Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,097	G→A	V397M (GTG→ATG)	ycjN →	putative ABC transporter periplasmic binding protein YcjN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,097	0	G	A	100.0%	101.0 / NA	28	V397M (GTG→ATG)	ycjN	putative ABC transporter periplasmic binding protein YcjN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

CAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGA  >  NC_000913/1373026‑1373169                                                                        |                                                                         cAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGtt                                                                 >  2:264364/1‑81 (MQ=255)  aaaTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTc                                                                <  1:264331/81‑1 (MQ=255)    atatTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCaa                                                              >  2:264378/1‑81 (MQ=255)     tatTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAAt                                                             >  2:264375/1‑81 (MQ=255)          aGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGc                                                        <  2:264332/81‑1 (MQ=255)               tttGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATaa                                                    <  2:264334/80‑1 (MQ=255)               tttGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATaa                                                    <  2:264333/80‑1 (MQ=255)                  ggCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGt                                                 >  2:264366/1‑80 (MQ=255)                     gcAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCAcc                                             >  2:264371/1‑81 (MQ=255)                           ggggATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGCGAGTTCAATGGTGCATAACGTCACCGTggg                                        >  2:264359/1‑80 (MQ=255)                             ggATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTa                                      >  2:264358/1‑80 (MQ=255)                              gATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTa                                      >  2:264383/1‑79 (MQ=255)                                  aaaaCTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGcc                                 <  1:264335/80‑1 (MQ=255)                                    aaCTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAt                              >  2:264374/1‑81 (MQ=255)                                     aCTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtc                             <  1:264337/81‑1 (MQ=255)                                     aCTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtc                             <  1:264336/81‑1 (MQ=255)                                      cTTTACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtct                            <  1:264338/81‑1 (MQ=255)                                       tttACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtctc                           >  2:264373/1‑81 (MQ=255)                                         tACCCGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtctc                           <  1:264339/79‑1 (MQ=255)                                            ccGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTctac                      >  1:264363/1‑81 (MQ=255)                                            ccGCATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTctac                      >  2:264370/1‑81 (MQ=255)                                               cATGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCtactac                   <  1:264340/81‑1 (MQ=255)                                                 tGGGTGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGc                 <  1:264341/81‑1 (MQ=255)                                                     tGATGTGACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGc              >  2:264369/1‑80 (MQ=255)                                                         gtgACGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGc          >  1:264379/1‑80 (MQ=255)                                                            aCGGGTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAg       <  2:264342/80‑1 (MQ=255)                                                                gTTCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAg   >  1:264368/1‑80 (MQ=255)                                                                 ttCTGGCATGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGa  >  2:264382/1‑80 (MQ=255)                                                                        |                                                                         CAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGA  >  NC_000913/1373026‑1373169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑