Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,411,109	A→T	D280E (GAT→GAA)	ttcA ←	tRNA cytosine(32) 2‑sulfurtransferase TtcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,411,109	0	A	T	100.0%	120.8 / NA	34	D280E (GAT→GAA)	ttcA	tRNA cytosine(32) 2‑sulfurtransferase TtcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/12);  total (22/12)

CAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCG  >  NC_000913/1411034‑1411184                                                                            |                                                                            cAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCaaa                                                                        >  2:270733/1‑81 (MQ=255) cAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCaa                                                                         <  1:270706/80‑1 (MQ=255)  aGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAAc                                                                       >  2:270743/1‑81 (MQ=255)     cGTAACTCATCCAACTGATTTCCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGcc                                                                    <  2:270707/81‑1 (MQ=255)        aaCTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGc                                                                     >  1:270765/1‑77 (MQ=255)          cTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGAt                                                                >  2:270750/1‑80 (MQ=255)            cATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAc                                                             >  2:270745/1‑81 (MQ=255)             aTCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAcc                                                            >  2:270730/1‑81 (MQ=255)             aTCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAc                                                             <  2:270708/80‑1 (MQ=255)                cAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAccccc                                                         >  2:270748/1‑81 (MQ=255)                cAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAcccc                                                          <  2:270709/80‑1 (MQ=255)                  aCTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGt                                                       >  2:270760/1‑81 (MQ=255)                            aTCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTc                                             <  2:270710/81‑1 (MQ=255)                                  ttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAcc                                       >  2:270756/1‑81 (MQ=255)                                    ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAcc                                       <  2:270711/79‑1 (MQ=255)                                    ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGt                                     >  2:270762/1‑81 (MQ=255)                                      ggCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg                                   >  2:270764/1‑81 (MQ=255)                                       gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGt                                     <  1:270712/78‑1 (MQ=255)                                       gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTggg                                  <  1:270713/81‑1 (MQ=255)                                        cTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGt                                 >  1:270758/1‑81 (MQ=255)                                           ccAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGAt                              <  1:270714/81‑1 (MQ=255)                                              gCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGcc                           >  2:270753/1‑81 (MQ=255)                                                aCGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCtt                         <  2:270715/81‑1 (MQ=255)                                                aCGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCtt                         >  2:270751/1‑81 (MQ=255)                                                    cGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGa                      >  2:270757/1‑80 (MQ=255)                                                        tGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATc                  >  1:270761/1‑80 (MQ=255)                                                         gTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCg                 >  1:270746/1‑80 (MQ=255)                                                         gTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCg                 >  2:270754/1‑80 (MQ=255)                                                           aGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa               >  1:270744/1‑80 (MQ=255)                                                             tGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAg            <  2:270716/81‑1 (MQ=255)                                                             tGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAg            >  2:270763/1‑81 (MQ=255)                                                                     ctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAt    >  2:270759/1‑81 (MQ=255)                                                                      ttCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTgg       <  1:270717/77‑1 (MQ=255)                                                                       tCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCg  >  1:270752/1‑81 (MQ=255)                                                                            |                                                                            CAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCG  >  NC_000913/1411034‑1411184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑