| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,452,220 | G→A | P475S (CCT→TCT) | paaZ ← | oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,452,220 | 0 | G | A | 96.2% | 87.2 / NA | 26 | P475S (CCT→TCT) | paaZ | oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/13); minor base T (1/0); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAA > NC_000913/1452148‑1452297 | cAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCAt > 1:277002/1‑81 (MQ=255) gCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATg > 2:276989/1‑80 (MQ=255) ggTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTAc > 2:276991/1‑81 (MQ=255) ggTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTAc < 2:276955/81‑1 (MQ=255) ttCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCa < 1:276956/81‑1 (MQ=255) cGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAg < 1:276957/80‑1 (MQ=255) gCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGtt > 2:276992/1‑81 (MQ=255) gCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGtt < 1:276959/81‑1 (MQ=255) gCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGt < 2:276958/80‑1 (MQ=255) aTGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGc < 2:276960/81‑1 (MQ=255) aTGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTg < 1:276961/80‑1 (MQ=255) ttCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGTCCCACCATGTACCAGTTGTGGCAGTGGGGAg > 2:277004/1‑81 (MQ=255) gTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCgg > 1:276994/1‑81 (MQ=255) aTTCTTCACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTg > 2:276998/1‑81 (MQ=255) ttcttcACCGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGc < 1:276962/81‑1 (MQ=255) ccGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGActct > 1:277006/1‑81 (MQ=255) cGCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCtt < 2:276963/81‑1 (MQ=255) gCCTCCTGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCtt > 1:277001/1‑80 (MQ=255) ctcctGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCAt > 1:277003/1‑81 (MQ=255) ctGCGCGACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTg < 1:276964/80‑1 (MQ=255) gcgACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGAt < 2:276965/81‑1 (MQ=255) cgACCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATc > 2:276995/1‑81 (MQ=255) aCCAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTg < 2:276966/81‑1 (MQ=255) ccAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGa < 1:276967/81‑1 (MQ=255) cAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGaa > 1:276997/1‑81 (MQ=255) cAGACCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGaa > 2:277010/1‑81 (MQ=255) | CAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAA > NC_000913/1452148‑1452297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |