Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,483,915	G→A	L285L (CTG→CTA)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,483,915	0	G	A	100.0%	104.6 / NA	29	L285L (CTG→CTA)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

CTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCT  >  NC_000913/1483837‑1483989                                                                               |                                                                           cTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAg                                                                          >  1:282337/1‑81 (MQ=255)    tCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGt                                                                         <  1:282299/79‑1 (MQ=255)    tCGCCCGATTGTTGAAGAAGCCGATGACACCAAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGt                                                                         <  1:282298/79‑1 (MQ=255)        ccGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGa                                                                    >  1:282340/1‑80 (MQ=255)         cGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGaaa                                                                  <  1:282300/81‑1 (MQ=255)                 gaagaaGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGc                                                          >  2:282341/1‑81 (MQ=255)                  aagaagCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCg                                                         <  1:282301/81‑1 (MQ=255)                   agaagCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGa                                                        >  2:282343/1‑81 (MQ=255)                       gCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACAtt                                                    <  1:282302/81‑1 (MQ=255)                          gATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCt                                                  >  1:282338/1‑80 (MQ=255)                           aTGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGa                                                >  1:282345/1‑81 (MQ=255)                            tGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGAt                                               <  1:282303/81‑1 (MQ=255)                             gACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACCAAGTCAGGAAAGCCATGGCGACATTCTGATc                                              >  2:282348/1‑81 (MQ=255)                                acCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCtt                                            >  1:282351/1‑80 (MQ=255)                                          aTCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAgcggcg                                 <  2:282305/81‑1 (MQ=255)                                          aTCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAgcggcg                                 <  2:282304/81‑1 (MQ=255)                                            cAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAg                               >  2:282344/1‑81 (MQ=255)                                                 gCAGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCggg                           >  2:282350/1‑80 (MQ=255)                                                   aGGCGATTTTTGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGaaa                        <  1:282306/81‑1 (MQ=255)                                                         tttttGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCcgcg                  <  2:282307/81‑1 (MQ=255)                                                          ttttGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGa                 >  1:282356/1‑81 (MQ=255)                                                            ttGACGCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGAt                <  1:282308/80‑1 (MQ=255)                                                                 gCCGTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATAccgcc          <  2:282309/81‑1 (MQ=255)                                                                    gTAGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGAt       <  2:282310/81‑1 (MQ=255)                                                                      aGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgc     >  1:282353/1‑81 (MQ=255)                                                                      aGACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgc     >  1:282349/1‑81 (MQ=255)                                                                       gACGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgc     <  2:282311/80‑1 (MQ=255)                                                                         cGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCt  >  1:282354/1‑81 (MQ=255)                                                                         cGAACTAAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCt  >  1:282352/1‑81 (MQ=255)                                                                               |                                                                           CTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCT  >  NC_000913/1483837‑1483989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑