Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,172,125	Δ1 bp	coding (749/1041 nt)	gatD ←	galactitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,172,124	0	G	.	100.0%	102.8 / NA	23	coding (750/1041 nt)	gatD	galactitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/9);  total (14/9)

AATATTTTGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTC  >  NC_000913/2172044‑2172198                                                                                 |                                                                           aaTATTTTGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCg                                                                            >  2:369483/1‑81 (MQ=255)   tatTTTGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCgc                                                                           >  2:369466/1‑79 (MQ=255)     ttttGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCgcaat                                                                        <  1:369434/81‑5 (MQ=255)      tttGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAAtc                                                                      >  2:369464/1‑81 (MQ=255)          ccAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATctct                                                                   <  1:369436/80‑1 (MQ=255)          ccAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATctct                                                                   <  2:369435/80‑1 (MQ=255)          ccAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTa                                                                  >  2:369467/1‑81 (MQ=255)           cAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTAc                                                                 >  1:369488/1‑81 (MQ=255)                                 cAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCgg                                            <  2:369437/80‑1 (MQ=255)                                  aGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTa                                          >  2:369474/1‑81 (MQ=255)                                      ccTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGc                                       >  2:369473/1‑80 (MQ=255)                                       cTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGcc                                      <  1:369439/80‑1 (MQ=255)                                       cTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCa                                     <  2:369438/81‑1 (MQ=255)                                            gCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGt                                >  1:369470/1‑81 (MQ=255)                                              aaCGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGtct                              >  2:369477/1‑81 (MQ=255)                                                cGTGCCCACCAGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCg                            <  1:369440/81‑1 (MQ=255)                                                       accaGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGAt                       >  1:369479/1‑79 (MQ=255)                                                          aGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCt                  >  1:369478/1‑81 (MQ=255)                                                          aGCGCCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCt                  >  1:369481/1‑81 (MQ=255)                                                             gcCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGAt               >  1:369472/1‑81 (MQ=255)                                                             gcCAGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGAt               <  2:369441/81‑1 (MQ=255)                                                                aGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTaa            <  1:369443/81‑1 (MQ=255)                                                                aGTTGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTaa            <  2:369442/81‑1 (MQ=255)                                                                  ttGGGCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAg          >  2:369482/1‑81 (MQ=255)                                                                    gggCATGAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAgcg        >  1:369471/1‑81 (MQ=255)                                                                          gAGGACC‑GCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTc  >  2:369476/1‑81 (MQ=255)                                                                                 |                                                                           AATATTTTGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTC  >  NC_000913/2172044‑2172198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑