Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,218	Δ1 bp	coding (115/2526 nt)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,215	0	C	.	100.0%	126.9 / NA	28	coding (118/2526 nt)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (16/12);  total (16/12)

CTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGC  >  NC_000913/309135‑309290                                                                                 |                                                                            cTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCAc                                                                             <  1:61061/81‑1 (MQ=255)  tGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCAcc                                                                            >  2:61095/1‑81 (MQ=255)  tGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCAcc                                                                            >  1:61097/1‑81 (MQ=255)    gCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCAccca                                                                          >  2:61094/1‑80 (MQ=255)    gCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCAccca                                                                          >  1:61102/1‑80 (MQ=255)             gagaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTtgc                                                                <  2:61062/81‑1 (MQ=255)                aTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTtgctgc                                                             >  2:61096/1‑81 (MQ=255)                aTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTtgctgc                                                             >  1:61109/1‑81 (MQ=255)                       taGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACtt                                                       <  1:61063/80‑1 (MQ=255)                            cGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGt                                                   >  2:61122/1‑79 (MQ=255)                            cGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTca                                                 <  1:61064/81‑1 (MQ=255)                                 cGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCagcagc                                            >  1:61111/1‑81 (MQ=255)                                  gCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAgcg                                           >  1:61131/1‑81 (MQ=255)                                    tCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCt                                         >  1:61115/1‑81 (MQ=255)                                          cGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGTTGCGCACTTATGTCAGCAGCGCTGGCATc                                   <  2:61066/81‑1 (MQ=255)                                          cGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATc                                   >  1:61099/1‑81 (MQ=255)                                          cGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATc                                   <  2:61065/81‑1 (MQ=255)                                              cTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGc                               >  2:61127/1‑81 (MQ=255)                                                 aaGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTg                             <  2:61067/80‑1 (MQ=255)                                                 aaGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTg                             >  1:61107/1‑80 (MQ=255)                                                  aGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTgaa                           >  1:61138/1‑81 (MQ=255)                                                  aGCGCCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTgaa                           >  2:61117/1‑81 (MQ=255)                                                     gcCTGACTGAAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTgaacga                        <  2:61068/81‑1 (MQ=255)                                                       ctgactgaAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTgaacgaa                       >  2:61101/1‑80 (MQ=255)                                                        tgactgaAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTgaacgaac                      >  1:61104/1‑80 (MQ=255)                                                         gactgaAGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACa                     >  1:61106/1‑80 (MQ=255)                                                              aaGGCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAg                <  2:61069/80‑1 (MQ=255)                                                                ggCCTGCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGAcc             <  1:61070/81‑1 (MQ=255)                                                                     gCGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGcc        >  2:61100/1‑81 (MQ=255)                                                                      cGGAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGcc        <  2:61071/80‑1 (MQ=255)                                                                        gAATAATCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCg       >  1:61114/1‑79 (MQ=255)                                                                         aataatCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAg    <  2:61072/81‑1 (MQ=255)                                                                           taatCA‑CCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGc  <  1:61073/81‑1 (MQ=255)                                                                                 |                                                                            CTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGC  >  NC_000913/309135‑309290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑