Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,994,202	(A)7→6	pseudogene (264/503 nt)	pbl →	transglycosylase domain‑containing protein Pbl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,994,196	0	A	.	100.0%	31.2 / NA	8	pseudogene (258/503 nt)	pbl	transglycosylase domain‑containing protein Pbl
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (3/5);  total (3/5)

AGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAG  >  NC_000913/2994122‑2994271                                                                           |                                                                            agagATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTaaaaaac                                                                       <  1:518079/81‑2 (MQ=255)      tAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGa                                                                 <  2:518080/81‑1 (MQ=255)                                attattGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGtt                                       <  1:518081/81‑1 (MQ=255)                                              cAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTc                         <  2:518082/81‑1 (MQ=255)                                                        aGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTa                >  2:518096/1‑80 (MQ=255)                                                           tGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTaaaa             >  1:518097/1‑80 (MQ=255)                                                                 ttCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAAc      >  1:518095/1‑81 (MQ=255)                                                                   ccccTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAAcc     <  1:518083/80‑1 (MQ=255)                                                                     ccTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCgag  <  2:518084/81‑1 (MQ=255)                                                                           |                                                                            AGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAG  >  NC_000913/2994122‑2994271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑