Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,429	Δ1 bp	coding (94/1668 nt)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,426	0	C	.	100.0%	110.9 / NA	25	coding (97/1668 nt)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/17);  total (8/17)

GATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCAT  >  NC_000913/4630346‑4630501                                                                                 |                                                                            gATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGc                                                                             <  1:854606/81‑1 (MQ=255)   tGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGccc                                                                           <  2:854607/81‑1 (MQ=255)             aaTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGgaagaa                                                                 <  1:854608/80‑1 (MQ=255)             aaTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGgaagaa                                                                 <  2:854609/80‑1 (MQ=255)             aaTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGaaa                                                                >  2:854638/1‑81 (MQ=255)                       aTAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCaga                                                          >  2:854633/1‑77 (MQ=255)                        tAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGAt                                                     <  1:854610/81‑1 (MQ=255)                        tAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGAt                                                     <  1:854611/81‑1 (MQ=255)                         aaTGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATg                                                    >  1:854646/1‑81 (MQ=255)                               cagcagGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTc                                              <  1:854612/81‑1 (MQ=255)                                 gcagGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCaa                                            <  1:854613/81‑1 (MQ=255)                                    gggTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCaaaa                                          >  2:854641/1‑80 (MQ=255)                                         gACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGa                                     <  1:854615/80‑1 (MQ=255)                                         gACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGAc                                    >  1:854645/1‑81 (MQ=255)                                         gACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGAc                                    >  1:854650/1‑81 (MQ=255)                                         gACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGAc                                    <  1:854614/81‑1 (MQ=255)                                                  gcgcCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcg                           <  1:854616/81‑1 (MQ=255)                                                  gcgcCATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcg                           >  1:854647/1‑81 (MQ=255)                                                  gagcCATTCAGACCCAGGAAACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcg                           <  1:854617/79‑1 (MQ=255)                                                      cATTCAGACCCAGGACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGaac                       <  1:854618/81‑1 (MQ=255)                                                                  ggACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGac           <  2:854619/81‑1 (MQ=255)                                                                  ggACACCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGa            >  2:854634/1‑80 (MQ=255)                                                                     cacCAATTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACg        <  1:854620/81‑1 (MQ=255)                                                                         aaTTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGc    <  2:854621/81‑1 (MQ=255)                                                                          aTTTTTG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCa   <  2:854622/81‑1 (MQ=255)                                                                           tttttG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCAt  <  2:854623/81‑1 (MQ=255)                                                                           tttttG‑CCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCAt  <  2:854624/81‑1 (MQ=255)                                                                                 |                                                                            GATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCAT  >  NC_000913/4630346‑4630501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑