Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,460,682	+T	coding (212/423 nt)	paaI →	phenylacetyl‑CoA thioesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,460,682	1	.	T	100.0%	102.9 / NA	31	Q71L (CAG→CTG)	paaI	phenylacetyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (23/8);  total (23/8)

GCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑GGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAA  >  NC_000913/1460602‑1460753                                                                                  |                                                                        gCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCa                                                                          >  2:258039/1‑81 (MQ=255)      aCGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑tgggg                                                                    <  2:258004/81‑2 (MQ=255)       cGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑tggggc                                                                   >  2:258026/1‑79 (MQ=255)        ggTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCt                                                                   >  1:258036/1‑81 (MQ=255)         gTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTTCAATAGCCATGGGGCTg                                                                  <  1:258005/81‑1 (MQ=255)         gTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTg                                                                  >  2:258030/1‑81 (MQ=255)            atcaAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTgg                                                                 >  2:258054/1‑79 (MQ=255)             tcaAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAg                                                              <  2:258006/81‑1 (MQ=255)              caAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGc                                                             >  1:258037/1‑81 (MQ=255)               aaaGTTGCCACGGCTGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGcc                                                            >  1:258072/1‑81 (MQ=255)               aaaGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGcc                                                            >  1:258038/1‑81 (MQ=255)                      ccACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCtt                                                     <  2:258008/81‑1 (MQ=255)                      ccACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCtt                                                     <  2:258007/81‑1 (MQ=255)                         cggcggGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTctg                                                  >  1:258028/1‑81 (MQ=255)                              ggCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTctgcctg                                              >  1:258035/1‑80 (MQ=255)                              ggCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTctgcctg                                              >  1:258034/1‑80 (MQ=255)                                 aGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCAc                                           >  1:258029/1‑80 (MQ=255)                                    tATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgatt                                       >  2:258050/1‑81 (MQ=255)                                    tATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgatt                                       >  2:258041/1‑81 (MQ=255)                                     aTTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgatt                                       >  1:258033/1‑80 (MQ=255)                                      ttttCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACgattga                                     >  2:258052/1‑81 (MQ=255)                                            cTGGCTAATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGAtttttt                               <  1:258009/81‑1 (MQ=255)                                               gCTGATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGc                             <  2:258010/80‑1 (MQ=255)                                                  gATACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTc                          >  2:258042/1‑80 (MQ=255)                                                    tACTGCCTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAg                       <  2:258011/81‑1 (MQ=255)                                                         ccTTTGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCttt                  <  2:258012/81‑1 (MQ=255)                                                             tGCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCg              >  2:258043/1‑81 (MQ=255)                                                              gCCTACGCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCg              >  2:258032/1‑80 (MQ=255)                                                                    gCCTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGacac       >  1:258046/1‑81 (MQ=255)                                                                     ccTGCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGA‑TTTTTGCGTCCAGGCTTTGCCGGAGACACCt     >  2:258049/1‑81 (MQ=255)                                                                        gCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCtt    >  1:258051/1‑80 (MQ=255)                                                                        gCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCtt    >  2:258057/1‑80 (MQ=255)                                                                        gCAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTCTTTGCGTCCAGGCTTTGCCGGAGACACCtt    >  2:258056/1‑80 (MQ=255)                                                                         cAATAGCCATGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTaa  >  1:258040/1‑81 (MQ=255)                                                                                  |                                                                        GCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCA‑GGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAA  >  NC_000913/1460602‑1460753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑