Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,567,095	+C	coding (46/1383 nt)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,567,094	1	.	C	100.0%	69.8 / NA	20	V16G (GTC→GGC)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (5/15);  total (5/15)

ACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGA‑CAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGC  >  NC_000913/1567017‑1567160                                                                               |                                                                   aCTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGA‑cca                                                                 <  2:275125/81‑3 (MQ=255)     gATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTc                                                              >  1:275157/1‑81 (MQ=255)       tACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCg                                                            <  1:275126/81‑1 (MQ=255)            aGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTcc                                                        <  2:275127/80‑1 (MQ=255)               tAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTc                                                    <  2:275128/81‑1 (MQ=255)                 aTGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCAt                                                  <  1:275129/81‑1 (MQ=255)                    aGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCtt                                               <  1:275130/81‑1 (MQ=255)                      cATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTc                                             <  2:275131/81‑1 (MQ=255)                       aTGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCa                                            >  1:275155/1‑81 (MQ=255)                             aaCGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCttt                                      >  1:275153/1‑81 (MQ=255)                                ggCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTaa                                   <  1:275132/81‑1 (MQ=255)                                     tttCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATAc                              <  1:275133/81‑1 (MQ=255)                                      ttCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACa                             >  1:275156/1‑81 (MQ=255)                                       tCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACa                             <  1:275134/80‑1 (MQ=255)                                        ccGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACAt                            <  1:275135/80‑1 (MQ=255)                                           cgGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTcc                        <  1:275136/81‑1 (MQ=255)                                              cTTTAGCACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCAt                      >  2:275158/1‑80 (MQ=255)                                                    cACGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTcac               <  1:275137/81‑1 (MQ=255)                                                      cGAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCacac             <  1:275138/81‑1 (MQ=255)                                                       gAATGGGCGGATCTGCCTGTTCGACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACAcc            <  2:275139/81‑1 (MQ=255)                                                                      ccTGTTCCACCAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGc  <  2:275140/76‑1 (MQ=255)                                                                               |                                                                   ACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGA‑CAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGC  >  NC_000913/1567017‑1567160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑