Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,628	+T	coding (652/1683 nt)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,625	1	.	T	100.0%	114.8 / NA	33	G219R (GGG→AGG)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (21/12);  total (21/12)

TTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCC‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTG  >  NC_000913/3120552‑3120702                                                                           |                                                                              ttAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCC‑ttttc                                                                          >  2:543105/1‑77 (MQ=255)    gagGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGc                                                                       <  1:543054/80‑1 (MQ=255)        tAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTcc                                                                  <  2:543055/81‑1 (MQ=255)           aGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatc                                                               <  2:543056/81‑1 (MQ=255)            gAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatca                                                              >  2:543093/1‑81 (MQ=255)            gAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatca                                                              <  2:543057/81‑1 (MQ=255)             aaCTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCcatcat                                                             >  1:543116/1‑81 (MQ=255)                tGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGc                                                          >  2:543099/1‑81 (MQ=255)                 gAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGc                                                          >  1:543095/1‑80 (MQ=255)                     gACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAc                                                       >  2:543112/1‑79 (MQ=255)                       cAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagc                                                   <  1:543058/81‑1 (MQ=255)                         gCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagcca                                                 <  2:543059/81‑1 (MQ=255)                           gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagccag                                                >  1:543101/1‑80 (MQ=255)                           gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagccag                                                >  2:543091/1‑80 (MQ=255)                           gAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTAccagccag                                                >  2:543082/1‑80 (MQ=255)                            aaGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGaa                                              <  2:543060/81‑1 (MQ=255)                              gCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACg                                            >  2:543110/1‑81 (MQ=255)                                 tCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGca                                         >  2:543124/1‑81 (MQ=255)                                 tCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGca                                         >  1:543089/1‑81 (MQ=255)                                  cccccAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGca                                         >  2:543103/1‑80 (MQ=255)                                                cgccgcTGGCCACGTCTCTTTCACCCCTTTTCCATCCTTCCATCATTTCTACCATCCATATCGTCTCAAGAACCCACAgg                           >  1:857792/1‑80 (MQ=255)                                                 gccgcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGaa                         >  1:543122/1‑81 (MQ=255)                                                  ccgcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAAc                        >  2:543100/1‑81 (MQ=255)                                                    gcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACgg                      >  1:543097/1‑81 (MQ=255)                                                    gcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACgg                      <  1:543061/81‑1 (MQ=255)                                                    gcTGGCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACgg                      >  1:543094/1‑81 (MQ=255)                                                       ggCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTaa                   >  1:543086/1‑81 (MQ=255)                                                       ggCCACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTaa                   >  2:543106/1‑81 (MQ=255)                                                         ccACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACt                 <  2:543062/81‑1 (MQ=255)                                                          cACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACt                 >  2:543090/1‑80 (MQ=255)                                                          cACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTg                <  2:543064/81‑1 (MQ=255)                                                          cACGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTg                <  1:543063/81‑1 (MQ=255)                                                            cGTCTCTTTCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGAc              >  1:543121/1‑81 (MQ=255)                                                                    tCACCCCTTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGc       <  1:543065/80‑1 (MQ=255)                                                                     acccct‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCAt    >  2:543108/3‑81 (MQ=255)                                                                      cccct‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCAtt   >  1:543128/2‑81 (MQ=255)                                                                       ccct‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTg  >  1:543117/1‑81 (MQ=255)                                                                           |                                                                              TTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCC‑TTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTG  >  NC_000913/3120552‑3120702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑