Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,358	(A)7→8	coding (228/693 nt)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,351	1	.	A	100.0%	83.8 / NA	25	A74E (GCA→GAA)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (15/10);  total (15/10)

CGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGT  >  NC_000913/3134274‑3134424                                                                               |                                                                          cggtcgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aa                                                                         >  2:545990/1‑80 (MQ=255) cggtcgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aa                                                                         >  2:545984/1‑80 (MQ=255)     cgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aaaaaa                                                                     >  2:545994/1‑80 (MQ=255)      gTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aaaaaaaa                                                                   >  2:546001/1‑80 (MQ=255)               cAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGa                                                           >  2:545996/1‑81 (MQ=255)                aaaaTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGAc                                                          <  2:545966/81‑1 (MQ=255)                  aaTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGAc                                                          >  2:545989/1‑79 (MQ=255)                   aTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAg                                                       >  2:545988/1‑81 (MQ=255)                      aCCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTc                                                    >  2:546007/1‑81 (MQ=255)                       ccGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTcc                                                   <  1:545968/81‑1 (MQ=255)                       ccGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTcc                                                   <  2:545967/81‑1 (MQ=255)                            gATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGgcgc                                              >  1:545995/1‑81 (MQ=255)                                 gAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTa                                         <  2:545969/81‑1 (MQ=255)                                          tACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCg                                >  2:546010/1‑81 (MQ=255)                                           aCATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGt                               >  1:546005/1‑81 (MQ=255)                                               aaaaCAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGt                           >  2:546011/1‑81 (MQ=255)                                                      ttGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGt                    <  2:545970/81‑1 (MQ=255)                                                      ttGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGt                    >  1:546002/1‑81 (MQ=255)                                                        gAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCg                  >  2:546014/1‑81 (MQ=255)                                                        gAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCg                  >  2:546017/1‑81 (MQ=255)                                                          aCGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCt                <  2:545971/81‑1 (MQ=255)                                                           cGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCtt               <  2:545972/81‑1 (MQ=255)                                                              aaTCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACt            >  1:546024/1‑81 (MQ=255)                                                                  aGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTCGTCGCCCGTCGCTTACTgcgc        >  2:546023/1‑81 (MQ=255)                                                                   ggTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCt       >  1:546019/1‑81 (MQ=255)                                                                    gTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCtt      <  2:545973/81‑1 (MQ=255)                                                                     tGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCttt     >  1:546009/1‑81 (MQ=255)                                                                       aaaaCAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTc    <  2:545974/80‑1 (MQ=255)                                                                        aaaCAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGt  <  2:545975/81‑1 (MQ=255)                                                                               |                                                                          CGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGT  >  NC_000913/3134274‑3134424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑