Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,299	+C	coding (166/474 nt)	smg ←	DUF494 domain‑containing protein Smg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,296	1	.	C	100.0%	68.8 / NA	20	L57V (TTG→GTG)	smg	DUF494 domain‑containing protein Smg
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (8/12);  total (8/12)

GCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑CCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCT  >  NC_000913/3432217‑3432366                                                                                 |                                                                       gCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑c                                                                       <  2:605733/81‑1 (MQ=255)   aTCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑ccc                                                                     <  1:605734/81‑1 (MQ=255)    tCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑cccc                                                                    >  1:605768/1‑80 (MQ=255)       aGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑ccccttc                                                                 <  1:605735/81‑5 (MQ=255)         tCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCt                                                                <  2:605736/81‑1 (MQ=255)                 acTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCa                                                        <  1:605737/81‑1 (MQ=255)                   tctTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAg                                                       >  2:605766/1‑80 (MQ=255)                     ttCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGc                                                      <  1:605738/79‑1 (MQ=255)                       ccGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCa                                                     <  2:605740/78‑1 (MQ=255)                       ccGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAg                                                   <  2:605739/80‑1 (MQ=255)                        cGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGt                                                  <  2:605741/80‑1 (MQ=255)                                 aaTACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGc                                         >  2:605764/1‑80 (MQ=255)                                    aCGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCa                                       >  1:605771/1‑79 (MQ=255)                                     cGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAg                                    <  2:605742/81‑1 (MQ=255)                                               gagGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTg                          >  2:605778/1‑81 (MQ=255)                                                agGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGt                         <  1:605743/81‑1 (MQ=255)                                                  gATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTg                          >  2:605783/1‑78 (MQ=255)                                                    tCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGa                      >  1:605772/1‑80 (MQ=255)                                                    tCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGa                      >  1:605765/1‑80 (MQ=255)                                                          gCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATAtcttc               <  1:605744/81‑1 (MQ=255)                                                                  cATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCa        <  2:605745/80‑1 (MQ=255)                                                                     cGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAAc    >  2:605775/1‑81 (MQ=255)                                                                      ggTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAAc    <  2:605746/80‑1 (MQ=255)                                                                       gTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCt  <  2:605747/81‑1 (MQ=255)                                                                                 |                                                                       GCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑CCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCT  >  NC_000913/3432217‑3432366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑