| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,084,127 | (T)5→6 | coding (1696/3051 nt) | fdoG ← | formate dehydrogenase O subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,084,122 | 1 | . | T | 93.9% | 107.1 / 1.3 | 33 | coding (1701/3051 nt) | fdoG | formate dehydrogenase O subunit alpha |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (17/14); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTG‑TTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATC > NC_000913/4084042‑4084197 | tGCCAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGATCTTCAGTTTCGACAGACAACCGATCACtttg > 2:741607/1‑81 (MQ=255) ccAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACtttg‑t < 2:741553/80‑1 (MQ=255) cAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACtttg‑ttt > 2:741626/1‑81 (MQ=255) aGTTAGAGGTTGCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGATAGACAACCGATCACtttg‑ttt‑tttg > 2:741622/1‑79 (MQ=255) ttAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACtttg‑ttt‑tttgtt > 1:741613/1‑77 (MQ=255) agagGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCg > 1:741612/1‑81 (MQ=255) agGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCggg < 1:741556/81‑1 (MQ=255) agGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCggg < 1:741557/81‑1 (MQ=255) gTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGaa < 2:741558/81‑1 (MQ=255) tcagtgttcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATg < 2:741559/80‑1 (MQ=255) tcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCaa > 2:741605/1‑79 (MQ=255) gTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTaa < 1:741560/81‑1 (MQ=255) cGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAg > 2:741606/1‑81 (MQ=255) gATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGc > 1:741604/1‑81 (MQ=255) tGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGccc > 1:741623/1‑81 (MQ=255) ggTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCt > 2:741619/1‑81 (MQ=255) gTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTg > 1:741609/1‑81 (MQ=255) tGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTgg > 2:741608/1‑81 (MQ=255) gACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTgg > 2:741616/1‑80 (MQ=255) aCGAGGAACTTCAGTTTCGACAGACAACCGGTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGc < 2:741561/80‑1 (MQ=38) cGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAg < 2:741562/81‑1 (MQ=255) gAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGata > 1:741621/1‑80 (MQ=255) cTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGc < 1:741563/81‑1 (MQ=255) cAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAt > 2:741615/1‑81 (MQ=255) aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt < 2:741566/81‑1 (MQ=255) aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 2:741596/1‑81 (MQ=255) aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt < 1:741565/81‑1 (MQ=255) aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 2:741614/1‑81 (MQ=255) aGTTTCGACAGACAACCGATCACTTTG‑TTTATTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAt < 2:741564/80‑1 (MQ=255) aGTTTCGACAGACAACCGATCACTTTG‑TTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 2:741632/1‑80 (MQ=255) gTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 1:741625/1‑80 (MQ=255) aaCCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCctct < 1:741567/81‑1 (MQ=255) cGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtc < 1:741568/81‑1 (MQ=255) aTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcat < 1:741569/81‑1 (MQ=255) aTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcat > 2:741617/1‑81 (MQ=255) aTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcat > 2:741634/1‑81 (MQ=255) tCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcatc < 2:741570/81‑1 (MQ=255) cTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCatcatc > 1:741630/1‑81 (MQ=255) | TGCCAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTG‑TTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATC > NC_000913/4084042‑4084197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |