Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,074	(A)7→8	intergenic (‑83/‑276)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,067	1	.	A	100.0%	26.7 / NA	9	intergenic (‑76/‑283)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (4/5);  total (4/5)

CTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCAC‑AAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAT  >  NC_000913/4312987‑4313135                                                                                  |                                                                     cTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTcac                                                                       <  2:790553/81‑1 (MQ=255)    atcaCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTcac‑a                                                                     <  2:790554/79‑1 (MQ=255)       aCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCAC‑aaaaaa                                                                <  1:790555/81‑1 (MQ=255)       aCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCAC‑aaaaaa                                                                <  1:790556/81‑1 (MQ=255)          ttCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCAC‑aaaaaaaat                                                             <  1:790557/81‑3 (MQ=255)                       gaattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATaa                                                   <  2:790559/79‑1 (MQ=255)                         attgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGtt                                               <  1:790560/81‑1 (MQ=255)                                  gCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGTttattttatt                                      <  2:790561/81‑1 (MQ=255)                                   ccACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGTTTATTTTATtg                                     >  1:790569/1‑81 (MQ=255)                                                     aaGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGc                   <  2:790562/81‑1 (MQ=255)                                                      aGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCt                  <  2:790563/81‑1 (MQ=255)                                                         aCAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTa                >  1:790564/1‑80 (MQ=255)                                                         aCAGAAAAGATGCTGGCGACTTCACAAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTa                >  1:790568/1‑80 (MQ=255)                                                                      tGGCGACTTCACAAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAt  >  1:790567/1‑81 (MQ=255)                                                                                  |                                                                     CTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCAC‑AAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAT  >  NC_000913/4312987‑4313135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑