| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,567,357 | +A | coding (1163/1233 nt) | mdtM ← | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,567,353 | 1 | . | A | 96.2% | 85.6 / ‑2.7 | 26 | coding (1167/1233 nt) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (10/15); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTG‑AAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCG > NC_000913/4567273‑4567427 | ggATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTg < 1:842270/81‑1 (MQ=255) aaaaTCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑acgacg < 1:842271/81‑1 (MQ=255) aaaaTCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑acgacg < 2:842273/81‑1 (MQ=255) aaaaTCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑acgacg < 2:842272/81‑1 (MQ=255) ctcctcCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAAcgcccg < 2:842274/81‑1 (MQ=255) cctccACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAAcgcccgc > 2:842314/1‑80 (MQ=255) cctccACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAAcgcccgc > 1:842306/1‑80 (MQ=255) ctccACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAAcgcccgc < 2:842276/79‑1 (MQ=255) ctccACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCca < 1:842275/81‑1 (MQ=255) cACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACaa < 2:842277/81‑1 (MQ=255) aGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGc > 1:842312/1‑81 (MQ=255) cGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTaa > 1:842309/1‑80 (MQ=255) ggCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACa < 1:842278/81‑1 (MQ=255) ggCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTG‑AAATACGACGATAACGCCCGCCACAACGGCTaa > 2:842313/1‑79 (MQ=255) gCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAg < 1:842279/81‑1 (MQ=255) tGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGAt > 2:842307/1‑81 (MQ=255) cTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGaa < 1:842280/81‑1 (MQ=255) tGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGc > 1:842317/1‑79 (MQ=255) tGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGc > 2:842310/1‑79 (MQ=255) gcgcACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAg < 1:842282/80‑1 (MQ=255) gcgcACGCGATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGc < 2:842281/81‑1 (MQ=255) cgATTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGAccgcc > 1:842316/1‑81 (MQ=255) aTTGAGCAATCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGt < 2:842283/81‑1 (MQ=255) aaTCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCa > 1:842301/1‑81 (MQ=255) aTCCCGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCa > 1:842320/1‑80 (MQ=255) ccGCCAGGGTGAAAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAg < 2:842284/80‑1 (MQ=255) aGGGTGAAAA‑ACGACGATACCGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCg < 2:842285/81‑1 (MQ=255) | GGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTG‑AAA‑ACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCG > NC_000913/4567273‑4567427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |