Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	574,490	+A	intergenic (+151/+39)	ybcQ → / ← nmpC	DLP12 prophage; putative antitermination protein/DLP12 prophage; putative outer membrane porin NmpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	574,489	1	.	A	100.0%	26.9 / NA	9	intergenic (+150/+40)	ybcQ/nmpC	DLP12 prophage; putative antitermination protein/DLP12 prophage; putative outer membrane porin NmpC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (7/2);  total (7/2)

GAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGG‑AGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCA  >  NC_000913/574416‑574565                                                                           |                                                                             gAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCaa                                                                        >  2:109777/1‑81 (MQ=255)  aaGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAAc                                                                       >  1:109780/1‑81 (MQ=255)   aGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAACg                                                                      <  2:109768/81‑1 (MQ=255)     gTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAACGcc                                                                    >  1:109778/1‑81 (MQ=255)                          gTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAACGCCTCCCGCAACATATCTTTTTCg                                               <  2:109769/81‑1 (MQ=255)                            tgtgCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAACGCCTCCCGCAACATATCTTTTTCGt                                              >  2:109781/1‑80 (MQ=255)                               gCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAACGCCTCCCGCAACATATCTTTTTCGTAAt                                           >  1:109779/1‑80 (MQ=255)                                       aGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAg                                   >  2:109785/1‑80 (MQ=255)                                                           cACTTATATGTTGGGGAAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTa                >  2:109786/1‑79 (MQ=255)                                                                    ttgggga‑AGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGt    >  2:109782/4‑81 (MQ=255)                                                                      gggga‑AGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGtca  >  2:109795/2‑81 (MQ=255)                                                                           |                                                                             GAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGG‑AGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCA  >  NC_000913/574416‑574565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑