| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,190,808 | 0 | C | T | 68.4% | 19.6 / 11.7 | 19 | G35R (GGA→AGA) | yehB | putative fimbrial usher protein YehB |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (4/9); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGATGTCATACTGCCCCGGTAAGGGTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCCACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGATTGCTG > NC_000913/2190734‑2190885 | atCGATGTCATACTGCCCCGGTAAGGGTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCTACCTAt > 2:372459/1‑81 (MQ=255) gATGTCATACTGCCCCGGTAAGGGTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCTACCTATCAt < 1:372441/81‑1 (MQ=255) tGCCCCGGTAAGGGTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCCACCTATCATAAAATGGGTa > 2:372461/1‑81 (MQ=255) ccccGGTAAGGGTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCCACCTATCATAAAATGGGTATc < 1:372442/81‑1 (MQ=255) ggTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTttct < 1:372443/81‑1 (MQ=255) tcaAGACGAATATTTGCAACCTGCTGGTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATaa < 1:372444/81‑1 (MQ=255) aaGACGAATATTTGCAACCTGCTGGTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAg < 2:372445/80‑1 (MQ=255) aaGACGAATATTTGCAACCTGCTGGTCTTTCATTCCACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGc < 1:692520/81‑1 (MQ=255) aGACGAATATTTGCAACCTGCTGGTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCt < 1:372446/81‑1 (MQ=255) aaTATTTGCAACCTGCTGGTCTTTCATTCCACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAAt > 2:372464/1‑81 (MQ=255) ccTGCTGGTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATa < 2:372447/81‑1 (MQ=255) ctgctgGTCTTTCATTCCACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATaa > 1:372469/1‑81 (MQ=255) ggTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTa > 2:372463/1‑81 (MQ=255) gTCTTTCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTAc < 1:372448/81‑1 (MQ=255) tttCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGAt > 1:372474/1‑81 (MQ=255) ttCATTCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGAtt > 1:372467/1‑81 (MQ=255) tCATTCTACCTATCATAAAATGGGTATCAAGGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGAtt < 2:372449/80‑1 (MQ=255) ttCTACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGATTGCTg < 2:372450/81‑1 (MQ=255) ttCCACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGATTGCTg < 2:372451/81‑1 (MQ=255) | ATCGATGTCATACTGCCCCGGTAAGGGTTGATTATCATCAAGACGAATATTTGCAACCTGCTGGTCTTTCATTCCACCTATCATAAAATGGGTATCAAAGGTTTCTTCAGCTGCATAAGCTTCAATGCCGAGCAATAACGCTACGATTGCTG > NC_000913/2190734‑2190885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |