| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,359,915 | 0 | C | T | 68.2% | 22.1 / 15.6 | 22 | A80T (GCC→ACC) | yfaV | putative transporter YfaV |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base T (10/5); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAG > NC_000913/2359836‑2359990 | ttCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGcc > 2:402480/1‑81 (MQ=255) tCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGccc > 2:402476/1‑81 (MQ=255) gCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGtccc > 1:402473/1‑80 (MQ=255) cTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGcccc > 1:402467/1‑79 (MQ=255) ccatgccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCaa < 1:402443/81‑1 (MQ=255) catgccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCaaa < 1:402444/81‑1 (MQ=255) gccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCaa > 1:402472/1‑77 (MQ=255) gCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGcc < 1:402445/81‑1 (MQ=255) gCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCgg > 2:402487/1‑80 (MQ=255) agaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACAcc > 2:402471/1‑80 (MQ=255) aaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACAcc > 2:402482/1‑78 (MQ=255) aTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGaa < 2:402446/81‑1 (MQ=255) cccACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACg < 2:402447/81‑1 (MQ=255) acaGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCat < 2:402448/81‑1 (MQ=255) acaGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCa > 1:402483/1‑80 (MQ=255) acaGCAGTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCa > 1:402477/1‑80 (MQ=255) agcagTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCatat < 1:402449/81‑1 (MQ=255) agTGTTGTCGTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATAcc > 2:402474/1‑81 (MQ=255) gTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGaa > 1:402492/1‑80 (MQ=255) gTACCAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGaa > 2:402484/1‑80 (MQ=255) gTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGaa > 1:402507/1‑80 (MQ=255) cAATCCAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGc < 2:402450/81‑1 (MQ=255) cAGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCt < 1:402451/81‑1 (MQ=255) aGGTTCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTc > 2:402491/1‑81 (MQ=255) ggTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTcc < 1:402452/81‑1 (MQ=255) tCTGGTCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAg > 2:402481/1‑81 (MQ=255) | TTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAG > NC_000913/2359836‑2359990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |