| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,362,667 | 0 | G | A | 64.3% | 18.6 / 24.9 | 28 | L323L (CTG→TTG) | yfaY | IPR008135 CinA family protein YfaY |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base A (6/12); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAC > NC_000913/2362592‑2362742 | gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCgg < 1:402919/81‑1 (MQ=255) gAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaa > 2:402960/1‑80 (MQ=255) aaGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAAt > 1:402979/1‑81 (MQ=255) tGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGt > 1:402954/1‑81 (MQ=255) ccGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTg > 2:402970/1‑81 (MQ=255) gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGc > 1:402956/1‑81 (MQ=255) gCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCccgcc < 2:402920/81‑1 (MQ=255) cGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCccgccg > 2:402957/1‑81 (MQ=255) ggCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTc > 2:402961/1‑81 (MQ=255) gcgcAAGGTTGAAATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTa < 2:402921/80‑1 (MQ=255) cgcAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAAt > 2:402973/1‑81 (MQ=255) aaGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAg > 2:402952/1‑81 (MQ=255) agaTGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcgc < 1:402924/80‑1 (MQ=255) agaTGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcgc < 1:402922/80‑1 (MQ=255) agaTGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcgc < 2:402923/80‑1 (MQ=255) gaTGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAg < 1:402925/81‑1 (MQ=255) cTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGttt < 2:402927/80‑1 (MQ=255) cTCGTTCTCGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTg < 2:402926/81‑1 (MQ=255) gTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgcgc < 1:402928/81‑1 (MQ=255) ctcGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg > 2:402953/1‑81 (MQ=255) ctcGAAACCCGAAACAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCa < 1:402929/80‑1 (MQ=255) aaaCCCGAAACAGCCATTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGtt < 2:402930/81‑1 (MQ=255) aCCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtc < 2:402931/81‑1 (MQ=255) aaCAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtg < 2:402932/81‑1 (MQ=255) aaCAGCCAGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtg > 2:402966/1‑81 (MQ=255) cAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgt > 1:402965/1‑80 (MQ=255) aGTGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGa < 2:402933/81‑1 (MQ=255) tGCCAACCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc < 1:402934/81‑1 (MQ=255) | GAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAC > NC_000913/2362592‑2362742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |