| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,377,490 | 0 | A | G | 63.3% | 21.7 / 27.0 | 30 | intergenic (‑70/+325) | sspA/rpsI | stringent starvation protein A/30S ribosomal subunit protein S9 |
| Reads supporting (aligned to +/- strand): ref base A (6/5); new base G (7/12); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTGGGCAGAAAATTGG > NC_000913/3377413‑3377567 | aCAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGccc < 1:594862/81‑1 (MQ=255) aGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGATACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCaa < 2:594863/81‑1 (MQ=255) ccATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATa < 2:594864/81‑1 (MQ=255) aTGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAg > 2:594901/1‑81 (MQ=255) aaaaCCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGaa < 2:594865/81‑1 (MQ=255) aaaaCCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGaa > 2:594898/1‑81 (MQ=255) aaaCCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAAc < 2:594866/81‑1 (MQ=255) aCCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGa > 2:594882/1‑81 (MQ=255) cTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGAct < 2:594867/81‑1 (MQ=255) cAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGActctc < 1:594869/81‑1 (MQ=255) cAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGActctc < 1:594868/81‑1 (MQ=255) gTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCg < 2:594870/81‑1 (MQ=255) aGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGaaa < 2:594871/81‑1 (MQ=255) aaTTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAAt > 1:594897/1‑81 (MQ=255) tttttACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATca < 1:594872/81‑1 (MQ=255) ttttACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATca > 2:594890/1‑80 (MQ=255) tttACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATcaaa > 1:594907/1‑81 (MQ=255) tACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATcaaac > 1:594896/1‑80 (MQ=255) aCTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATcaaaca < 2:594873/80‑1 (MQ=255) cTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATcaaacaaa < 1:594874/81‑1 (MQ=255) cTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATcaaacaa > 2:594899/1‑80 (MQ=255) cTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACaaaa < 2:594875/79‑1 (MQ=255) aCCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAAt > 2:594893/1‑80 (MQ=255) ggTGGCCAGTCAGAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCg < 2:594876/81‑1 (MQ=255) gTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGa > 2:594895/1‑81 (MQ=255) gTCAGAAGTTGTGTTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTgg > 2:594894/1‑81 (MQ=255) gAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTggg > 2:594900/1‑78 (MQ=255) gAAGTTGTGTTGCCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTGGGCAg < 2:594877/81‑1 (MQ=255) gtTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTTGGCAGAAAATTg > 1:594904/1‑80 (MQ=255) ttACCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTGGGCAGAAAATTgg < 2:594878/80‑1 (MQ=255) | ACAGCCATGAAAACCTCCAGGTATAGTCAGAATTTTTACTGCTACCAGCCACCAGGTGGCCAGTCAGAAGTTGTGTTACCCAATAAGGAACGACTCTCTTTGTTCGAAAATCAAACAAAAAATGAGCAATACCCGACATTTGGGCAGAAAATTGG > NC_000913/3377413‑3377567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |