Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,4160CT74.1% 38.4 / 14.6 27V64V (GTG→GTA) kefBK(+) : H(+) antiporter KefB
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (13/7);  total (17/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATC  >  NC_000913/3480340‑3480491
                                                                            |                                                                           
cGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAAtactac                                                                           <  2:615326/79‑1 (MQ=255)
 gCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGcc                                                                        >  1:615360/1‑81 (MQ=255)
    aaaaaTCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAg                                                                     >  1:615352/1‑81 (MQ=255)
        aTCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTcc                                                                 >  1:615356/1‑81 (MQ=255)
           gAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGa                                                               >  1:615362/1‑80 (MQ=255)
             aCGCCGCAGTTGCCAAGGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGaa                                                              >  1:615376/1‑79 (MQ=255)
               gccgcAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGt                                                          <  1:615327/81‑1 (MQ=255)
                  gcAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTg                                                         >  2:615366/1‑79 (MQ=255)
                  gcAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTg                                                         >  1:615373/1‑79 (MQ=255)
                    aGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggagg                                                     <  2:615328/81‑1 (MQ=255)
                        gCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGAtc                                                  <  2:615329/80‑1 (MQ=255)
                           aaaGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCAt                                              >  2:615372/1‑81 (MQ=255)
                            aaGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATc                                             <  1:615330/81‑1 (MQ=255)
                            aaGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATc                                             >  2:615386/1‑81 (MQ=255)
                             aGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATc                                             >  2:615369/1‑80 (MQ=255)
                                  ggAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                        <  1:615331/80‑1 (MQ=255)
                                     gggATTCAACTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCt                                    <  1:615332/81‑1 (MQ=255)
                                            aaCTCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAAc                             <  1:615334/81‑1 (MQ=255)
                                            aaCTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATaaa                              <  1:615333/80‑1 (MQ=255)
                                            aaCCCAAGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAAc                             >  1:615370/1‑81 (MQ=255)
                                                  aGGCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGc                       <  2:615335/81‑1 (MQ=255)
                                                    gCCGATGATAAACATCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGccc                     >  1:615374/1‑81 (MQ=255)
                                                     ccGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGcccc                    >  1:615389/1‑81 (MQ=255)
                                                           atAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACgg               <  1:615336/80‑1 (MQ=255)
                                                                 aTCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAAtt        >  1:615367/1‑81 (MQ=255)
                                                                 aTCAGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAAtt        >  2:615371/1‑81 (MQ=255)
                                                                    aGGAATACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGc      >  1:615379/1‑80 (MQ=255)
                                                                       aaTACTACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATc  >  1:615382/1‑81 (MQ=255)
                                                                            |                                                                           
CGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATC  >  NC_000913/3480340‑3480491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑