| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,800,601 | 0 | A | G | 61.9% | 14.3 / 17.0 | 21 | I122T (ATC→ACC) | waaY | lipopolysaccharide core heptose (II) kinase |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (8/5); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGCTGAATTTTATTTTTTAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGT > NC_000913/3800527‑3800671 | attgCTGAATTTTATTTTTTAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTGATCa > 2:681609/1‑81 (MQ=255) aaTTTTATTTTTTAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGatat > 2:681605/1‑80 (MQ=255) aaTTTTATTTTTTAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGata < 2:681586/79‑1 (MQ=255) tttattttTTAGCGCATCATCAATATCGGGCATATCACACACCTCTATGCCATCAATATACTCGGTGATCATGATATAAGt > 1:681607/1‑81 (MQ=255) attttTTAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATg > 1:681613/1‑81 (MQ=255) ttttttAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATgg < 1:681587/81‑1 (MQ=255) ttttttAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATg > 1:681604/1‑80 (MQ=255) tttAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTTATCATGATATAAGTATGGAc < 2:681588/80‑1 (MQ=255) ttAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATGGACa > 2:681612/1‑80 (MQ=255) tAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACaa < 2:681589/80‑1 (MQ=255) aGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATGGACaaaa > 2:681617/1‑81 (MQ=255) aGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATGGACaaaa > 1:681608/1‑81 (MQ=255) gcgcATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAAc < 2:681590/81‑1 (MQ=255) gggCATATCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTc < 2:681591/81‑1 (MQ=255) gCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAg < 2:681592/81‑1 (MQ=255) tCACACAACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCaat < 1:681593/81‑1 (MQ=255) cacacaACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCaat > 1:681615/1‑80 (MQ=255) caACTCTATGCCATCAATATACTCGGTGATCATGATATAAGTATGGACATAACGTAAGGTTTTCCGTTCAGCCaataaat > 2:681610/1‑80 (MQ=255) tATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAg < 2:681594/79‑1 (MQ=255) aTGCCATCAATATACTCGGTGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCa > 2:681611/1‑81 (MQ=255) aaTATACTCGGTGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAgtgt < 1:681595/81‑1 (MQ=255) | ATTGCTGAATTTTATTTTTTAGCGCATCATCAATATCGGGCATATCACACAACTCTATGCCATCAATATACTCGATGATCATGATATAAGTATGGACAAAACGTAAGGTTTTCCGTTCAGCCAATAAATAAAAGTCATTGAGTGT > NC_000913/3800527‑3800671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |