Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,087	C→T	C243Y (TGC→TAC)	hcp ←	protein S‑nitrosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,087	0	C	T	100.0%	98.8 / NA	29	C243Y (TGC→TAC)	hcp	protein S‑nitrosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/11);  total (18/11)

TAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGA  >  NC_000913/913009‑913164                                                                               |                                                                              tAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTAt                                                                             <  1:169644/81‑1 (MQ=255)   gACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTAtt                                                                            <  1:169645/80‑1 (MQ=255)      aTTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTAcc                                                                        >  2:169695/1‑81 (MQ=255)            gCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGt                                                                  >  2:169680/1‑81 (MQ=255)              ccGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCg                                                                >  2:169665/1‑81 (MQ=255)                gTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCt                                                              >  2:169678/1‑81 (MQ=255)                  gCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCttt                                                            >  1:169671/1‑81 (MQ=255)                      tCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGACGCTTTGACg                                                        >  2:169670/1‑81 (MQ=255)                        ggTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGt                                                       <  2:169646/80‑1 (MQ=255)                         gTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGtt                                                      >  1:169682/1‑80 (MQ=255)                                    cagGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTgg                                          <  1:169647/81‑1 (MQ=255)                                     agGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGc                                         >  1:169673/1‑81 (MQ=255)                                     agGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGc                                         >  1:169689/1‑81 (MQ=255)                                          gTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCgg                                    >  1:169677/1‑81 (MQ=255)                                          gTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCg                                     <  1:169648/80‑1 (MQ=255)                                            agagATCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGt                                  >  2:169674/1‑81 (MQ=255)                                             gagaTCTTTGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTg                                 <  2:169649/81‑1 (MQ=255)                                                     tGAGATCGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTAt                          <  1:169650/80‑1 (MQ=255)                                                           cGTGACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCgg                   >  1:169693/1‑81 (MQ=255)                                                              gACCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGttt                >  2:169676/1‑81 (MQ=255)                                                               aCCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTc               <  2:169651/81‑1 (MQ=255)                                                               aCCGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTc               >  1:169687/1‑81 (MQ=255)                                                                 cGGAAATCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGc             >  1:169675/1‑81 (MQ=255)                                                                    aaaTCAGAATGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTg          <  1:169652/81‑1 (MQ=255)                                                                       gcAGAATGTATTGACCCGCCGTCGCTTTGACGTTGGCCTGGGTTGGCGTCGGGTGACCGTATGTACCGGTTTCGCCTGCAt       <  2:169653/80‑1 (MQ=255)                                                                           aaTGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATc      >  1:169684/1‑78 (MQ=255)                                                                           aaTGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATc      >  1:169681/1‑78 (MQ=255)                                                                            aTGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAg   >  1:169686/1‑80 (MQ=255)                                                                            aTGTATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGa  <  2:169654/81‑1 (MQ=255)                                                                               |                                                                              TAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGA  >  NC_000913/913009‑913164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑