Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	59,243	A→G	K41K (AAA→AAG)	yabQ →	protein YabQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	59,243	0	A	G	100.0%	50.3 / NA	15	K41K (AAA→AAG)	yabQ	protein YabQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/7);  total (8/7)

AAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCT  >  NC_000913/59167‑59315                                                                             |                                                                         aaaTCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTAc                                                                      >  2:11905/1‑81 (MQ=255)   aTCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTACt                                                                     >  1:11914/1‑80 (MQ=255)    tCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGa                                                                   >  1:11908/1‑81 (MQ=255)      ccccccAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGAc                                                                  <  1:11892/80‑1 (MQ=255)                    tGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGaa                                                   >  1:11906/1‑81 (MQ=255)                         cTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAg                                               <  1:11893/80‑1 (MQ=255)                                        cTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAgg                               >  1:11907/1‑81 (MQ=255)                                              aagTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAg                         >  1:11915/1‑81 (MQ=255)                                               agTTGCTACTAAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAgg                        <  1:11894/81‑1 (MQ=255)                                                    ctactaAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTg                   <  1:11895/81‑1 (MQ=255)                                                      actaAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTgg                  >  1:11910/1‑80 (MQ=255)                                                       ctaAAAAAGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTgg                  <  1:11896/79‑1 (MQ=255)                                                          aaaaaGTACTGCTGAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAAc               <  1:11897/79‑1 (MQ=255)                                                                  ctgctgAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCaa     <  1:11898/81‑1 (MQ=255)                                                                      tgAACAAGTTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCt  >  1:11917/1‑80 (MQ=255)                                                                             |                                                                         AAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCT  >  NC_000913/59167‑59315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑