Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,032,971	C→T	T278I (ACA→ATA)	hyaA →	hydrogenase 1 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,032,971	0	C	T	100.0%	97.2 / NA	28	T278I (ACA→ATA)	hyaA	hydrogenase 1 small subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

CGCTGCCCGCAAAGGTTACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCACACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGCGGAAAATGGTTT  >  NC_000913/1032897‑1033043                                                                           |                                                                         cGCTGCCCGCAAAGGTTACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTg                                                                    >  2:190079/1‑81 (MQ=255)      cccGCAAAGGTTACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAatg                                                               <  2:190057/81‑1 (MQ=255)         gCAAAGGTTACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAatgatg                                                            <  2:190058/81‑1 (MQ=255)            aaGGTTACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTGTAACGCCTGTTCCTCCATACGCTGGAATGATGGCg                                                         >  2:190102/1‑81 (MQ=255)                 tACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGtttct                                                    >  2:190092/1‑81 (MQ=255)                      ccTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTcc                                                >  1:190093/1‑80 (MQ=255)                         gTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAAt                                            >  1:190108/1‑81 (MQ=255)                           aCAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATc                                           >  1:190101/1‑80 (MQ=255)                            cAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATcc                                          <  1:190059/80‑1 (MQ=255)                            cAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATcc                                          <  2:190060/80‑1 (MQ=255)                               aaTGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTc                                      >  2:190104/1‑81 (MQ=255)                                aTGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCt                                     >  2:190105/1‑81 (MQ=255)                                     cTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCa                                <  2:190061/81‑1 (MQ=255)                                     cTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCa                                <  2:190062/81‑1 (MQ=255)                                     cTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCa                                <  1:190063/81‑1 (MQ=255)                                     cTGCAAAGGGCCTACCACCTATAACGCCTGTACCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCa                                <  2:190064/81‑1 (MQ=255)                                        cAAAGGGCCTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACg                              <  1:190065/80‑1 (MQ=255)                                                cTACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTgg                     >  1:190099/1‑81 (MQ=255)                                                 tACCACCTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTggg                    >  1:190113/1‑81 (MQ=255)                                                   ccaccTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCt                  >  2:190089/1‑81 (MQ=255)                                                    caccTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCtg                 <  2:190066/81‑1 (MQ=255)                                                      ccTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCtgtg               <  2:190067/81‑1 (MQ=255)                                                      ccTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCtgtg               >  2:190097/1‑81 (MQ=255)                                                       cTATAACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGc              <  1:190068/81‑1 (MQ=255)                                                         ataACGCCTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGCg             >  1:190106/1‑80 (MQ=255)                                                                cTGTTCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGCGGAAAATgg     >  1:190098/1‑81 (MQ=255)                                                                   ttCCTCCATACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGCGGAAAATGGttt  <  2:190070/81‑1 (MQ=255)                                                                   ttCCTCCATACGCTGGAATGATGGCGTTTCTATCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGCGGAAAATGGttt  <  2:190069/81‑1 (MQ=255)                                                                           |                                                                         CGCTGCCCGCAAAGGTTACTGCCTGTACAAAATGGGCTGCAAAGGGCCTACCACCTATAACGCCTGTTCCTCCACACGCTGGAATGATGGCGTTTCTTTCCCAATCCAGTCTGGTCACGGCTGCCTGGGCTGTGCGGAAAATGGTTT  >  NC_000913/1032897‑1033043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑