Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,647	A→G	S65P (TCG→CCG)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,647	0	A	G	100.0%	95.0 / NA	27	S65P (TCG→CCG)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/11);  total (16/11)

AAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCG  >  NC_000913/1069571‑1069721                                                                             |                                                                           aaaGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCAt                                                                         >  1:196350/1‑80 (MQ=255)     ccGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTaaaa                                                                    <  2:196328/81‑1 (MQ=255)        cgcTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATgg                                                                 <  2:196329/81‑1 (MQ=255)         gcTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATgg                                                                 >  2:196363/1‑80 (MQ=255)         gcTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATgg                                                                 >  2:196351/1‑80 (MQ=255)           tGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGa                                                                >  2:196369/1‑79 (MQ=255)            ggAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGa                                                                >  2:196376/1‑78 (MQ=255)                       aaCTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATc                                                    >  1:196365/1‑79 (MQ=255)                               aCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCAt                                           >  1:196366/1‑80 (MQ=255)                               aCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCAt                                           >  2:196374/1‑80 (MQ=255)                                    cccgccGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAAt                                     <  2:196330/81‑1 (MQ=255)                                        ccgGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGc                                 <  1:196331/81‑1 (MQ=255)                                         cgGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCa                                >  2:196361/1‑81 (MQ=255)                                         cgGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCa                                <  1:196332/81‑1 (MQ=255)                                          ggTAATAAAGAAAAACAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCAt                               <  1:196333/81‑1 (MQ=255)                                                  aGAAAAACAGCAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCg                       >  1:196379/1‑81 (MQ=255)                                                         cAGTAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGcc                <  1:196334/81‑1 (MQ=255)                                                            tAACGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCaaa             <  2:196335/81‑1 (MQ=255)                                                             aaCGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCaaa             >  2:196368/1‑80 (MQ=255)                                                             aaCGTGCCGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCaaa             >  2:196375/1‑80 (MQ=255)                                                                    cGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCa     <  1:196336/81‑1 (MQ=255)                                                                    cGATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCa     >  2:196370/1‑81 (MQ=255)                                                                     gATCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCAc    >  1:196373/1‑81 (MQ=255)                                                                      aTCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCAcc   >  1:196364/1‑81 (MQ=255)                                                                      aTCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCAcc   <  2:196337/81‑1 (MQ=255)                                                                      aTCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCAcc   >  1:196377/1‑81 (MQ=255)                                                                       tCCCCGGCATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACcg  <  2:196338/81‑1 (MQ=255)                                                                             |                                                                           AAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCG  >  NC_000913/1069571‑1069721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑