Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,393	A→T	F183L (TTT→TTA)	opgC ←	protein required for succinyl modification of osmoregulated periplasmic glucans

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,393	0	A	T	100.0%	28.2 / NA	10	F183L (TTT→TTA)	opgC	protein required for succinyl modification of osmoregulated periplasmic glucans
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/6);  total (4/6)

GAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCT  >  NC_000913/1108329‑1108469                                                                 |                                                                             gAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAATAAAATCACCGATAGtt                                                              <  1:202306/81‑1 (MQ=255) gAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAATAAAATCACCGATAGtt                                                              <  2:202307/81‑1 (MQ=255)           aTACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAATAAAATCACCGATAGTTTTACCATCga                                                    <  1:202308/81‑1 (MQ=255)            tACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAATAAAATCACCGATAGTTTTACCATc                                                      >  1:202319/1‑78 (MQ=255)                   tAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAATAAAATCACCGATAGTTTTACCATCGAGAAttttt                                            <  2:202309/81‑1 (MQ=255)                                      taCCGCATAACCGATGCCGAGGCATAATAAAATCACCGATAGTTTTACAATCGAGAATTTTTTATTCGTTTTATCAGAAtt                         >  2:202318/1‑81 (MQ=255)                                          gCATAACCGATGCCGAGGCATAATAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCt                     <  2:202310/81‑1 (MQ=255)                                              aaCCGATGCCGAGGCATAATAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAat                 >  2:202324/1‑81 (MQ=255)                                                      ccGAGGCATAATAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCt          <  1:202311/80‑1 (MQ=255)                                                       cGAGGCATAATAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGa        >  2:202322/1‑81 (MQ=255)                                                             ataataAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCt  >  1:202320/1‑81 (MQ=255)                                                                 |                                                                             GAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCT  >  NC_000913/1108329‑1108469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑