Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,437	A→G	R174R (CGT→CGC)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,437	0	A	G	100.0%	136.3 / NA	38	R174R (CGT→CGC)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

GCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGG  >  NC_000913/81361‑81514                                                                             |                                                                              gcagcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGt                                                                            <  1:16603/80‑1 (MQ=255)   agcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCg                                                                         <  2:16604/81‑1 (MQ=255)    gcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGa                                                                        <  1:16605/81‑1 (MQ=255)       cGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGAtct                                                                     <  1:16606/81‑1 (MQ=255)                tATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGt                                                            >  1:16646/1‑81 (MQ=255)                 atcgatcgACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGt                                                            <  1:16607/80‑1 (MQ=255)                  tcgatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGa                                                          >  2:16653/1‑81 (MQ=255)                  tcgatcgaCGTCACTCTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGa                                                          >  2:16661/1‑81 (MQ=255)                    gatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGAt                                                         <  2:16608/80‑1 (MQ=255)                       cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATacac                                                     >  2:16640/1‑81 (MQ=255)                            tCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCgg                                                <  1:16609/81‑1 (MQ=255)                                 ttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATc                                            >  1:16649/1‑80 (MQ=255)                                    tgGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCaaa                                         <  1:16610/80‑1 (MQ=255)                                        gACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCtt                                    <  2:16611/81‑1 (MQ=255)                                        gACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCtt                                    >  1:16647/1‑81 (MQ=255)                                           gCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCttttt                                 <  1:16612/81‑1 (MQ=255)                                            cTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTc                                <  1:16613/81‑1 (MQ=255)                                               gCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCata                             >  1:16644/1‑81 (MQ=255)                                                cGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCatat                            <  2:16614/81‑1 (MQ=255)                                                cGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCata                             <  1:16615/80‑1 (MQ=255)                                                 gAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATAtt                           >  1:16655/1‑81 (MQ=255)                                                     aGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTcc                       <  1:16616/81‑1 (MQ=255)                                                      gATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCg                      >  2:16648/1‑81 (MQ=255)                                                       aTTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGc                     >  1:16650/1‑81 (MQ=255)                                                        ttCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCt                    <  2:16617/81‑1 (MQ=255)                                                          cAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTa                   <  1:16618/80‑1 (MQ=255)                                                            aaCGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCt                <  2:16619/81‑1 (MQ=255)                                                                cgATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgc            <  2:16620/81‑1 (MQ=255)                                                                 gATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg           >  2:593226/1‑81 (MQ=255)                                                                 gATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg           >  2:16662/1‑81 (MQ=255)                                                                 gATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg           <  1:16621/81‑1 (MQ=255)                                                                   tGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGc         >  2:16656/1‑81 (MQ=255)                                                                      gggCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCtt      >  1:16660/1‑81 (MQ=255)                                                                       ggCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCttt     <  2:16622/81‑1 (MQ=255)                                                                       ggCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCttt     >  1:16643/1‑81 (MQ=255)                                                                        gCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCtttt    <  1:16623/81‑1 (MQ=255)                                                                          gATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTgg  >  2:16645/1‑81 (MQ=255)                                                                          gATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTgg  >  2:16657/1‑81 (MQ=255)                                                                             |                                                                              GCAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGG  >  NC_000913/81361‑81514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑