| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,113,489 | G→A | intergenic (+83/‑90) | opgH → / → yceK | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H/DUF1375 domain‑containing lipoprotein YceK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,113,489 | 0 | G | A | 94.4% | 53.1 / ‑3.6 | 18 | intergenic (+83/‑90) | opgH/yceK | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H/DUF1375 domain‑containing lipoprotein YceK |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (10/7); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACG > NC_000913/1113415‑1113565 | ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAgg > 1:203198/1‑81 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGc < 1:203181/80‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCAc < 1:203182/81‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCAc > 1:203197/1‑81 (MQ=255) tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCAcg < 2:203183/81‑1 (MQ=255) cTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAaacaa > 2:203213/1‑81 (MQ=255) gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGcc > 1:203205/1‑80 (MQ=255) cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATa < 1:203184/80‑1 (MQ=255) gCAATTTATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATaaa > 1:203212/1‑80 (MQ=255) tttATTGATTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATAc > 2:203202/1‑81 (MQ=255) aTTTTGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGt > 1:203214/1‑80 (MQ=255) ttttGCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTa < 1:203185/81‑1 (MQ=255) gCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGcc > 2:203218/1‑81 (MQ=255) gCAAGACTTTGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc < 2:203186/80‑1 (MQ=255) tttGTGGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttt > 1:203206/1‑81 (MQ=255) tgtgGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttt > 2:203207/1‑81 (MQ=255) tgtgGGTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttt < 2:203187/80‑1 (MQ=255) gTCGAATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACg < 1:203188/81‑1 (MQ=255) | TTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACG > NC_000913/1113415‑1113565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |